Dynamic elements govern the catalytic activity of CapE, a capsular polysaccharide-synthesizing enzyme from Staphylococcus aureus

Takamitsu Miyafusa, Jose M.M. Caaveiro, Yoshikazu Tanaka, Kouhei Tsumoto

研究成果: ジャーナルへの寄稿記事

9 引用 (Scopus)

抄録

CapE is an essential enzyme for the synthesis of capsular polysaccharide (CP) of pathogenic strains of Staphylococcus aureus. Herein we demonstrate that CapE is a 5-inverting 4,6-dehydratase enzyme. However, in the absence of downstream enzymes, CapE catalyzes an additional reaction (5-back-epimerization) affording a by-product under thermodynamic control. Single-crystal X-ray crystallography was employed to identify the structure of the by-product. The structural analysis reveals a network of coordinated motions away from the active site governing the enzymatic activity of CapE. A second dynamic element (the latch) regulates the enzymatic chemoselectivity. The validity of these mechanisms was evaluated by site-directed mutagenesis.

元の言語英語
ページ(範囲)3824-3830
ページ数7
ジャーナルFEBS Letters
587
発行部数23
DOI
出版物ステータス出版済み - 11 29 2013
外部発表Yes

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Polysaccharides
Staphylococcus aureus
Catalyst activity
Byproducts
Enzymes
Hydro-Lyases
Mutagenesis
X ray crystallography
X Ray Crystallography
Site-Directed Mutagenesis
Thermodynamics
Structural analysis
Catalytic Domain
Single crystals

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

これを引用

Dynamic elements govern the catalytic activity of CapE, a capsular polysaccharide-synthesizing enzyme from Staphylococcus aureus. / Miyafusa, Takamitsu; Caaveiro, Jose M.M.; Tanaka, Yoshikazu; Tsumoto, Kouhei.

:: FEBS Letters, 巻 587, 番号 23, 29.11.2013, p. 3824-3830.

研究成果: ジャーナルへの寄稿記事

Miyafusa, Takamitsu ; Caaveiro, Jose M.M. ; Tanaka, Yoshikazu ; Tsumoto, Kouhei. / Dynamic elements govern the catalytic activity of CapE, a capsular polysaccharide-synthesizing enzyme from Staphylococcus aureus. :: FEBS Letters. 2013 ; 巻 587, 番号 23. pp. 3824-3830.
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