Dynamic Equilibrium and Heterogeneity of Mouse Pluripotent Stem Cells with Distinct Functional and Epigenetic States

Katsuhiko Hayashi, Susana M.Chuva de Sousa Lopes, Fuchou Tang, M. Azim Surani

研究成果: Contribution to journalArticle査読

486 被引用数 (Scopus)

抄録

Embryonic stem cells (ESCs) are apparently homogeneous self-renewing cells, but we observed heterogeneous expression of Stella in ESCs, which is a marker of pluripotency and germ cells. Here we show that, whereas Stella-positive ESCs were like the inner cell mass (ICM), Stella-negative cells were like the epiblast cells. These states were interchangeable, which reflects the metastability and plasticity of ESCs. The established equilibrium was skewed reversibly in the absence of signals from feeder cells, which caused a marked shift toward an epiblast-like state, while trichostatin A, an inhibitor of histone deactelylase, restored Stella-positive population. The two populations also showed different histone modifications and striking functional differences, as judged by their potential for differentiation. The Stella-negative ESCs were more like the postimplantation epiblast-derived stem cells (EpiSCs), albeit the stella locus was repressed by DNA methylation in the latter, which signifies a robust epigenetic boundary between ESCs and EpiSCs.

本文言語英語
ページ(範囲)391-401
ページ数11
ジャーナルCell stem cell
3
4
DOI
出版ステータス出版済み - 10 9 2008
外部発表はい

All Science Journal Classification (ASJC) codes

  • 分子医療
  • 遺伝学
  • 細胞生物学

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