Dynamics of the carbohydrate chains attached to the Fc portion of immunoglobulin G as studied by NMR spectroscopy assisted by selective 13C labeling of the glycans

Yoshiki Yamaguchi, Koichi Kato, Mitsuru Shindo, Shin Aoki, Kumiko Furusho, Kenji Koga, Noriko Takahashi, Yoji Arata, Ichio Shimada

研究成果: Contribution to journalArticle

49 引用 (Scopus)

抜粋

A systematic method for 13C labeling of the glycan of immunoglobulin G for NMR study has been developed. A mouse immunoglobulin of subclass IgG2b has been used for the experiment. On the basis of chemical shift and linewidth data, it has been concluded that (1) the mobility of the carbohydrate chain in IgG2b is comparable to that of the backbone polypeptide chain with the exception of the galactose residue at the nonreducing end of the Manα1-3 branch, which is extremely mobile and (2) agalactosylation does not induce any significant change in the mobility. The results obtained indicate that even in the agalactosyl form the glycans are buried in the protein. Biological significance of the NMR results obtained is also briefly discussed.

元の言語英語
ページ(範囲)385-394
ページ数10
ジャーナルJournal of Biomolecular NMR
12
発行部数3
DOI
出版物ステータス出版済み - 1998

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Spectroscopy

フィンガープリント Dynamics of the carbohydrate chains attached to the Fc portion of immunoglobulin G as studied by NMR spectroscopy assisted by selective <sup>13</sup>C labeling of the glycans' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用