Dysfunctional and short-lived subsets in monocyte-derived dendritic cells from patients with advanced cancer

Hideya Ohnishi, Takashi Morisaki, Eishi Baba, Hirotaka Kuga, Hideo Kuroki, Kotaro Matsumoto, Masao Tanaka, Mitsuo Katano

研究成果: ジャーナルへの寄稿記事

37 引用 (Scopus)

抄録

Dendritic cells (DCs) are antigen-presenting cells specialized for the induction of the primary T-cell response. Tumor immunotherapy using DCs loaded with tumor antigens is under way for patients with several types of advanced malignancies. In this study, DC-like cells (Mo-DCs) were generated from peripheral blood monocytes with granulocyte-macrophage colony-stimulating factor and interleukin-4. The antigen-presenting abilities, including capture of apoptotic tumor cells, IL-12 secretion, expression of antigen-presentation-related molecules (HLA-ABC, HLA-DR, and CD80), and mixed leukocyte reaction, of Mo-DCs from 37 patients with advanced cancer (pMo-DCs) were compared to those of 20 healthy volunteers (hMo-DCs). Seven days after the initial culture, no significant difference was found in either the number or the size of Mo-DC-forming colonies between the two groups. However, most of the antigen-presenting abilities of pMo-DCs were weaker than those of hMo-DCs on day 7. On day 14, both number and size of colonies were significantly decreased in pMo-DCs but not in hMo-DCs. Interestingly, the antigen-presenting abilities of the remaining pMo-DCs gradually strengthened with time and by day 14 no significant difference was observed between pMo-DCs and hMo-DCs. These results indicate that pMo-DCs contain dysfunctional and short-lived Mo-DC subsets.

元の言語英語
ページ(範囲)286-295
ページ数10
ジャーナルClinical Immunology
105
発行部数3
DOI
出版物ステータス出版済み - 12 1 2002

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Dendritic Cells
Monocytes
Neoplasms
Antigens
Mixed Lymphocyte Culture Test
Antigen Presentation
HLA-DR Antigens
Neoplasm Antigens
Antigen-Presenting Cells
Interleukin-12
Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-4
Immunotherapy
Healthy Volunteers

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

これを引用

Dysfunctional and short-lived subsets in monocyte-derived dendritic cells from patients with advanced cancer. / Ohnishi, Hideya; Morisaki, Takashi; Baba, Eishi; Kuga, Hirotaka; Kuroki, Hideo; Matsumoto, Kotaro; Tanaka, Masao; Katano, Mitsuo.

:: Clinical Immunology, 巻 105, 番号 3, 01.12.2002, p. 286-295.

研究成果: ジャーナルへの寄稿記事

Ohnishi, Hideya ; Morisaki, Takashi ; Baba, Eishi ; Kuga, Hirotaka ; Kuroki, Hideo ; Matsumoto, Kotaro ; Tanaka, Masao ; Katano, Mitsuo. / Dysfunctional and short-lived subsets in monocyte-derived dendritic cells from patients with advanced cancer. :: Clinical Immunology. 2002 ; 巻 105, 番号 3. pp. 286-295.
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