E-cadherin regulates proliferation of colorectal cancer stem cells through NANOG

Shingo Tamura, Taichi Isobe, Hiroshi Ariyama, Michitaka Nakano, Yoshikane Kikushige, Shigeo Takaishi, Hitoshi Kusaba, Katsuto Takenaka, Takashi Ueki, Masafumi Nakamura, Koichi Akashi, Eishi Baba

研究成果: Contribution to journalArticle

5 被引用数 (Scopus)

抄録

Cancer stem cells (CSCs) possess a self-renewal ability and display tumorigenic potential in immunodeficient mice. Colorectal CSCs are thought to be a uniform population and no functionally distinct subpopulations have been identified. Because E-cadherin is an essential molecule for self-renewal of embryonic stem cells, we examined E-cadherin expression, which may play a role in maintaining the properties of CSCs, in EpCAMhigh/CD44+ colorectal CSCs from human primary colorectal cancers. We obtained 18 surgical specimens of human primary colorectal cancer. CD44, EpCAM, and E-cadherin expression were analyzed by fluorescence-activated cell sorting. Sorted EpCAMhigh/CD44+ colorectal CSCs were injected into immunodeficient mice to estimate the tumorigenic potential. Genetic profiles were analyzed by cDNA microarray. Notably, colorectal CSCs could be divided into two populations based on the E-cadherin expression status, and they exhibited different pathological characteristics. Compared to E-cadherin-negative colorectal CSCs, E-cadherin-positive (EC+) colorectal CSCs demonstrated higher tumor growth potential in vivo. EC+ colorectal CSCs revealed a higher expression of the pluripotency factor NANOG, which contributed to the higher tumor growth potential of EC+ colorectal CSCs through control of cyclin D1 expression. These findings are the first demonstration of functionally distinct subpopulations of colorectal CSCs in human clinical samples.

本文言語英語
ページ(範囲)693-703
ページ数11
ジャーナルOncology reports
40
2
DOI
出版ステータス出版済み - 8 2018

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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