Early pregnancy diagnosis based on luteal morphology and blood flow on Days 17–21 post-artificial insemination in Japanese Black cattle

Tomomi Kanazawa, Motohide Seki, Kosuke Iga

    研究成果: ジャーナルへの寄稿学術誌査読

    抄録

    The present study was conducted to evaluate the applicability of corpus luteum (CL) morphology and blood flow for early pregnancy diagnosis on Days 17–21 post-artificial insemination (AI) in Japanese Black (JB) cattle. Ultrasound examinations were performed on 230 inseminated JB cattle (202 cows and 28 heifers) to evaluate CL morphological characteristics [CL area, CL tissue area, presence/absence of a central cavity, and central cavity area (CCA)] and blood flow area (BFA) on Days 17 (n = 46), 18 (n = 45), 19 (n = 46), 20 (n = 47), and 21 (n = 46) (Day 0 = the day of AI). Pregnancy was confirmed using brightness-mode ultrasonography on Day 30. In pregnant group (n = 141), CL area and CL tissue area were greater (P < 0.001) than those in non-pregnant group (n = 89) on and after Day 19. Moreover, BFA was greater (P < 0.05) in pregnant group on and after Day 18. The overall central cavity incidence was lower (P < 0.001) and CCA was smaller (P < 0.001) in pregnant group. Logistic regression analyses revealed that the best single predictor for pregnancy diagnosis was BFA on Days 17–20 (sensitivity = 69%–100% and specificity = 65%–91%). On Day 21, CL area or CL tissue area was the most accurate predictor (cutoff value = 267.78 mm2, sensitivity = 100%, and specificity = 100%). The best set of predictors for pregnancy diagnosis was specified as the BFA and [CCA × BFA] pair. Setting BFA cutoff value in the cattle without central cavity at 55.26 mm2 yielded the highest sensitivity (97%) and specificity (88%) on Days 18–20. The accuracy of pregnancy diagnosis using BFA or BFA and [CCA × BFA] pair on Day 17 was lower than those on and after Day 18. In conclusion, BFA and CCA are effective for early pregnancy diagnosis on Days 18–20 post-AI in JB cattle; however, it is inadequate on Day 17 owing to its low accuracy. Evaluating CL area or CL tissue area on Day 21 is the most accurate method of pregnancy diagnosis.

    本文言語英語
    ページ(範囲)69-78
    ページ数10
    ジャーナルTheriogenology
    181
    DOI
    出版ステータス出版済み - 3月 15 2022

    !!!All Science Journal Classification (ASJC) codes

    • 小型動物
    • 食用動物
    • 動物科学および動物学
    • 馬獣医学

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