Effect of acute imipramine administration on the pattern of forced swim-induced c-Fos expression in the mouse brain

Satoru Yanagida, Keisuke Motomura, Ayako Ohashi, Kentaro Hiraoka, Tomofumi Miura, Shigenobu Kanba

研究成果: ジャーナルへの寄稿記事

6 引用 (Scopus)

抄録

The forced swim test (FST) has been widely used for the preclinical evaluation of antidepressant drugs. Despite considerable differences in the protocol, equivalence of the FST for rats and mice has been rarely questioned. Previous research on the FST for rats revealed that repeated administration of antidepressant drugs attenuates the c-Fos response to swim stress in the hypothalamus and limbic regions. However, few studies have made similar investigations using the FST for mice. In the present study, we explored the mouse brain through immunohistochemistry staining for c-Fos after acute administration of imipramine or saline with or without a subsequent swim session. Imipramine enhanced the c-Fos density in regions of the central extended amygdala, while forced swim stress increased c-Fos expression in some hypothalamic (the ventrolateral preoptic nucleus and dorsomedial nucleus) and brain stem regions, which is consistent with previous reports. In contrast to previous literature with rats, swim stress brought a significant increase in c-Fos expression in the lateral septal nucleus and some other regions in the hypothalamus (the intermediate hypothalamic area, the paraventricular and arcuate nucleus) only in the imipramine-pretreated group, which has not been observed previously. In the arcuate nucleus, double immunostaining revealed that c-Fos was rarely co-expressed with proopiomelanocortin or tyrosine hydroxylase regardless of imipramine treatment. The present results suggest that the activation of several regions in the lateral septum and the hypothalamus underlies antidepressant-like effect in the mouse FST.

元の言語英語
ページ(範囲)119-124
ページ数6
ジャーナルNeuroscience Letters
629
DOI
出版物ステータス出版済み - 8 26 2016

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Imipramine
Antidepressive Agents
Arcuate Nucleus of Hypothalamus
Brain
Hypothalamus
Lateral Hypothalamic Area
Mediodorsal Thalamic Nucleus
Septal Nuclei
Pro-Opiomelanocortin
Preoptic Area
Paraventricular Hypothalamic Nucleus
Tyrosine 3-Monooxygenase
Brain Stem
Immunohistochemistry
Staining and Labeling
Research
Therapeutics

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

これを引用

Effect of acute imipramine administration on the pattern of forced swim-induced c-Fos expression in the mouse brain. / Yanagida, Satoru; Motomura, Keisuke; Ohashi, Ayako; Hiraoka, Kentaro; Miura, Tomofumi; Kanba, Shigenobu.

:: Neuroscience Letters, 巻 629, 26.08.2016, p. 119-124.

研究成果: ジャーナルへの寄稿記事

Yanagida, Satoru ; Motomura, Keisuke ; Ohashi, Ayako ; Hiraoka, Kentaro ; Miura, Tomofumi ; Kanba, Shigenobu. / Effect of acute imipramine administration on the pattern of forced swim-induced c-Fos expression in the mouse brain. :: Neuroscience Letters. 2016 ; 巻 629. pp. 119-124.
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