Effect of aluminum on metabolism of organic acids and chemical forms of aluminum in root tips of Eucalyptus camaldulensis Dehnh.

Takashi Ikka, Tsuyoshi Ogawa, Donghua Li, Syuntaro Hiradate, Akio Morita

研究成果: Contribution to journalArticle査読

20 被引用数 (Scopus)

抄録

Eucalyptus (Eucalyptus camaldulensis) has relatively high resistance to aluminum (Al) toxicity than the various herbaceous plants and model plant species. To investigate Al-tolerance mechanism, the metabolism of organic acids and the chemical forms of Al in the target site (root tips) in Eucalyptus was investigated. To do this, 2-year old rooted cuttings of E. camaldulensis were cultivated in half-strength Hoagland solution (pH 4.0) containing Al (0, 0.25, 0.5, 1.0, 2.5 and 5.0 mM) salts for 5 weeks; growth was not affected at concentrations up to 2.5 mM even with Al concentration reaching 6000 μg g1 DW. In roots, the citrate content also increased with increasing Al application. Concurrently, the activities of aconitase and NADP +-isocitrate dehydrogenase, which catalyze the decomposition of citrate, decreased. On the other hand, the activity of citrate synthase was not affected at concentrations up to 2.5 mM Al. 27Al-NMR spectroscopic analyses were carried out where it was found that Al-citrate complexes were a major chemical form present in cell sap of root tips. These findings suggested that E. camaldulensis detoxifies Al by forming Al-citrate complexes, and that this is achieved through Al-induced citrate accumulation in root tips via suppression of the citrate decomposition pathway.

本文言語英語
ページ(範囲)142-147
ページ数6
ジャーナルPhytochemistry
94
DOI
出版ステータス出版済み - 2013
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学
  • 植物科学
  • 園芸学

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