Effect of antibiotics on extracellular protein level in Pseudomonas aeruginosa

研究成果: Contribution to journalArticle査読

4 被引用数 (Scopus)

抄録

Pseudomonas aeruginosa PAO1 organisms harbouring different plasmids were cultured in broths containing appropriate antibiotic(s). Extracellular proteins were more abundant in the presence of tetracycline or kanamycin than in the presence of other antibiotics. Zymography revealed that alkaline protease (AprA) production was interfered by these antibiotics. Extracellular proteins were not observed at the same level when AprA-deficient EG03 strains were cultured in the presence of different antibiotics. The extracellular protein levels were dependent on the antibiotics and plasmid derivative groups. Levels of extracellular protein were not significantly different between PAO1 (pBBR1MCS-5) and EG03 (pAprcomp-MCS5), and profiles of the extracellular proteome were comparable. In contrast, the level of EG03 (pBBR1MCS-MCS5) extracellular protein was higher than those observed in the other two strains. These results suggested that although AprA partially contributes to the alteration of extracellular protein level, the effect is limited.

本文言語英語
ページ(範囲)44-50
ページ数7
ジャーナルPlasmid
84-85
DOI
出版ステータス出版済み - 3 1 2016

All Science Journal Classification (ASJC) codes

  • 分子生物学

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