Effect of antibiotics on extracellular protein level in Pseudomonas aeruginosa

Eigo Takahashi, Man Lee, Hiroaki Mon, Yuuka Chieda, Chisa Yasunaga-Aoki, Takahiro Kusakabe, Kazuhiro Iiyama

研究成果: ジャーナルへの寄稿記事

2 引用 (Scopus)

抄録

Pseudomonas aeruginosa PAO1 organisms harbouring different plasmids were cultured in broths containing appropriate antibiotic(s). Extracellular proteins were more abundant in the presence of tetracycline or kanamycin than in the presence of other antibiotics. Zymography revealed that alkaline protease (AprA) production was interfered by these antibiotics. Extracellular proteins were not observed at the same level when AprA-deficient EG03 strains were cultured in the presence of different antibiotics. The extracellular protein levels were dependent on the antibiotics and plasmid derivative groups. Levels of extracellular protein were not significantly different between PAO1 (pBBR1MCS-5) and EG03 (pAprcomp-MCS5), and profiles of the extracellular proteome were comparable. In contrast, the level of EG03 (pBBR1MCS-MCS5) extracellular protein was higher than those observed in the other two strains. These results suggested that although AprA partially contributes to the alteration of extracellular protein level, the effect is limited.

元の言語英語
ページ(範囲)44-50
ページ数7
ジャーナルPlasmid
84-85
DOI
出版物ステータス出版済み - 3 1 2016

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Pseudomonas aeruginosa
Anti-Bacterial Agents
Proteins
Plasmids
Kanamycin
Proteome
Tetracycline
alkaline protease

All Science Journal Classification (ASJC) codes

  • Molecular Biology

これを引用

Effect of antibiotics on extracellular protein level in Pseudomonas aeruginosa. / Takahashi, Eigo; Lee, Man; Mon, Hiroaki; Chieda, Yuuka; Yasunaga-Aoki, Chisa; Kusakabe, Takahiro; Iiyama, Kazuhiro.

:: Plasmid, 巻 84-85, 01.03.2016, p. 44-50.

研究成果: ジャーナルへの寄稿記事

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abstract = "Pseudomonas aeruginosa PAO1 organisms harbouring different plasmids were cultured in broths containing appropriate antibiotic(s). Extracellular proteins were more abundant in the presence of tetracycline or kanamycin than in the presence of other antibiotics. Zymography revealed that alkaline protease (AprA) production was interfered by these antibiotics. Extracellular proteins were not observed at the same level when AprA-deficient EG03 strains were cultured in the presence of different antibiotics. The extracellular protein levels were dependent on the antibiotics and plasmid derivative groups. Levels of extracellular protein were not significantly different between PAO1 (pBBR1MCS-5) and EG03 (pAprcomp-MCS5), and profiles of the extracellular proteome were comparable. In contrast, the level of EG03 (pBBR1MCS-MCS5) extracellular protein was higher than those observed in the other two strains. These results suggested that although AprA partially contributes to the alteration of extracellular protein level, the effect is limited.",
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AU - Takahashi, Eigo

AU - Lee, Man

AU - Mon, Hiroaki

AU - Chieda, Yuuka

AU - Yasunaga-Aoki, Chisa

AU - Kusakabe, Takahiro

AU - Iiyama, Kazuhiro

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AB - Pseudomonas aeruginosa PAO1 organisms harbouring different plasmids were cultured in broths containing appropriate antibiotic(s). Extracellular proteins were more abundant in the presence of tetracycline or kanamycin than in the presence of other antibiotics. Zymography revealed that alkaline protease (AprA) production was interfered by these antibiotics. Extracellular proteins were not observed at the same level when AprA-deficient EG03 strains were cultured in the presence of different antibiotics. The extracellular protein levels were dependent on the antibiotics and plasmid derivative groups. Levels of extracellular protein were not significantly different between PAO1 (pBBR1MCS-5) and EG03 (pAprcomp-MCS5), and profiles of the extracellular proteome were comparable. In contrast, the level of EG03 (pBBR1MCS-MCS5) extracellular protein was higher than those observed in the other two strains. These results suggested that although AprA partially contributes to the alteration of extracellular protein level, the effect is limited.

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