Effect of coffee on the compositional shift of oral indigenous microbiota cultured in vitro

Koji Ogata, Toru Takeshita, Yukie Shibata, Rie Matsumi, Shinya Kageyama, Mikari Asakawa, Yoshihisa Yamashita

研究成果: Contribution to journalArticle

抄録

Coffee is a widely consumed beverage containing organic compounds with antibacterial activity. To investigate its possible effect on the growth of oral indigenous microbiota, saliva samples collected from nine young adults were inoculated into brain heart infusion (BHI) medium with or without addition of coffee compounds and cultured at 37°C in 5% CO2 for 12 h. The total bacterial density and composition after cultivation for 0, 6, and 12 h were determined by quantitative PCR analysis and 16S rRNA gene sequencing, respectively. The increase in total bacterial load was significantly inhibited by addition of the coffee compounds. The microbiota was mostly composed of Streptococcus species after culture in BHI medium regardless of the addition of coffee compounds. The proportion of Streptococcus salivarius was significantly reduced after addition of coffee relative to that in untreated medium alone, whereas the proportions of Streptococcus mitis and Streptococcus infantis were increased. These results suggest that exposure to coffee affects the composition of the oral Streptococcus population, in addition to inhibiting the overall growth of salivary bacteria. Considered in the light of data from earlier epidemiological studies, it is possible to conclude that coffee consumption contributes to better health.

元の言語英語
ページ(範囲)418-424
ページ数7
ジャーナルJournal of Oral Science
61
発行部数3
DOI
出版物ステータス出版済み - 9 2019

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

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