Effect of deglycosylation of N-linked sugar chains on glucose oxidase from Aspergillus niger.

K. Takegawa, K. Fujiwara, S. Iwahara, K. Yamamoto, T. Tochikura

研究成果: Contribution to journalArticle査読

50 被引用数 (Scopus)

抄録

Endo-beta-N-acetylglucosaminidase from Flavobacterium sp. released about 30% of the N-linked sugar chains from the glucose oxidase of Aspergillus niger. To elucidate the role of the carbohydrate moiety, the enzymatic properties of native and carbohydrate-depleted glucose oxidases were compared. It was found that their catalytic activities and thermal and pH stabilities were identical. However, the carbohydrate-depleted glucose oxidase was more rapidly precipitated by the addition of trichloroacetic acid and ammonium sulfate than the native enzyme. These results show that the N-linked sugar chains of the glucose oxidase contributed to the high solubility of the enzyme in water.

本文言語英語
ページ(範囲)460-464
ページ数5
ジャーナルBiochemistry and cell biology = Biochimie et biologie cellulaire
67
8
DOI
出版ステータス出版済み - 1 1 1989
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学
  • 細胞生物学

フィンガープリント

「Effect of deglycosylation of N-linked sugar chains on glucose oxidase from Aspergillus niger.」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル