Effect of DNA length on dielectrophoretic characteristics of DNA-labeled microbeads

Hiromichi Kasahara, Zhenhao Ding, Michihiko Nakano, Junya Suehiro

研究成果: 会議への寄与タイプ論文

3 引用 (Scopus)

抄録

Polymerase chain reaction (PCR) is a powerful tool for diagnostic procedures in bacterial and viral infections. We have developed a new electrical technique for rapid detection of DNA amplified by PCR using dielectrophoresis (DEP) of microbeads that are chemically labeled with the amplicons. The DNA immobilization on the microbeads alters their DEP behavior in such a way that they are trapped on a microelectrode under the action of positive DEP, whereas pristine microbeads are not. Combining the dramatic alteration in DEP characteristics with impedance measurement leads to rapid and quantitative detection of amplicons. The method is based on the surface conductivity dependence of microbeads DEP characteristics. It was expected that the surface conductivity would depend on the length of DNA fragments immobilized on a microbeads. In this study, it was found that the crossover frequency was dependent on the length of DNA.

元の言語英語
ページ3341-3346
ページ数6
DOI
出版物ステータス出版済み - 6 16 2015
イベント2015 IEEE International Conference on Industrial Technology, ICIT 2015 - Seville, スペイン
継続期間: 3 17 20153 19 2015

その他

その他2015 IEEE International Conference on Industrial Technology, ICIT 2015
スペイン
Seville
期間3/17/153/19/15

Fingerprint

Electrophoresis
DNA
Polymerase chain reaction
Microelectrodes

All Science Journal Classification (ASJC) codes

  • Computer Science Applications
  • Electrical and Electronic Engineering

これを引用

Kasahara, H., Ding, Z., Nakano, M., & Suehiro, J. (2015). Effect of DNA length on dielectrophoretic characteristics of DNA-labeled microbeads. 3341-3346. 論文発表場所 2015 IEEE International Conference on Industrial Technology, ICIT 2015, Seville, スペイン. https://doi.org/10.1109/ICIT.2015.7125593

Effect of DNA length on dielectrophoretic characteristics of DNA-labeled microbeads. / Kasahara, Hiromichi; Ding, Zhenhao; Nakano, Michihiko; Suehiro, Junya.

2015. 3341-3346 論文発表場所 2015 IEEE International Conference on Industrial Technology, ICIT 2015, Seville, スペイン.

研究成果: 会議への寄与タイプ論文

Kasahara, H, Ding, Z, Nakano, M & Suehiro, J 2015, 'Effect of DNA length on dielectrophoretic characteristics of DNA-labeled microbeads' 論文発表場所 2015 IEEE International Conference on Industrial Technology, ICIT 2015, Seville, スペイン, 3/17/15 - 3/19/15, pp. 3341-3346. https://doi.org/10.1109/ICIT.2015.7125593
Kasahara H, Ding Z, Nakano M, Suehiro J. Effect of DNA length on dielectrophoretic characteristics of DNA-labeled microbeads. 2015. 論文発表場所 2015 IEEE International Conference on Industrial Technology, ICIT 2015, Seville, スペイン. https://doi.org/10.1109/ICIT.2015.7125593
Kasahara, Hiromichi ; Ding, Zhenhao ; Nakano, Michihiko ; Suehiro, Junya. / Effect of DNA length on dielectrophoretic characteristics of DNA-labeled microbeads. 論文発表場所 2015 IEEE International Conference on Industrial Technology, ICIT 2015, Seville, スペイン.6 p.
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