Effect of ENU (ethylnitrosourea) mutagenesis in cleft lip and/or palate pathogenesis in mice

Tomohiro Yamada, K. Fujiwara, K. Mishima, T. Sugahara

研究成果: ジャーナルへの寄稿記事

4 引用 (Scopus)

抄録

Cleft lip and/or palate (CL/P) are caused by many factors. The aim of this study was to investigate the effects of genetic point mutations in CL/P pathogenesis. ICR and AJ strain mice were used. Ethylnitrosourea (ENU) was injected into 10-week-old male mice (G0) intraperitoneally at a dose of 250 mg/kg. The males were bred with two untreated virgin females of the same strain on day 100 after injection. The uterine contents (G1) of one female were examined on day 18.5 of pregnancy. From the other female, the offspring were delivered naturally, and F3 mice (G3) were also examined in the same way. In ICR strain mice, cleft palate only (CPO) was increased in both the G1 and G3. The frequency was significantly higher in the G3 than the G1 generation. Cleft lip was not observed. In AJ strain mice, CL/P increased in both the G1 and G3. In the G3, the frequency of CPO was increased significantly. Genes related to CPO may be recessive in phenotype. CPO and cleft lip differ from a genetic viewpoint. Point mutations play a significant role in cleft lip and palate.

元の言語英語
ページ(範囲)74-77
ページ数4
ジャーナルInternational Journal of Oral and Maxillofacial Surgery
34
発行部数1
DOI
出版物ステータス出版済み - 1 1 2005

Fingerprint

Ethylnitrosourea
Cleft Lip
Cleft Palate
Mutagenesis
Point Mutation
Inbred ICR Mouse
Phenotype
Pregnancy
Injections

All Science Journal Classification (ASJC) codes

  • Surgery
  • Oral Surgery
  • Otorhinolaryngology

これを引用

Effect of ENU (ethylnitrosourea) mutagenesis in cleft lip and/or palate pathogenesis in mice. / Yamada, Tomohiro; Fujiwara, K.; Mishima, K.; Sugahara, T.

:: International Journal of Oral and Maxillofacial Surgery, 巻 34, 番号 1, 01.01.2005, p. 74-77.

研究成果: ジャーナルへの寄稿記事

@article{e4181a74531d447dbcb20f8be999a411,
title = "Effect of ENU (ethylnitrosourea) mutagenesis in cleft lip and/or palate pathogenesis in mice",
abstract = "Cleft lip and/or palate (CL/P) are caused by many factors. The aim of this study was to investigate the effects of genetic point mutations in CL/P pathogenesis. ICR and AJ strain mice were used. Ethylnitrosourea (ENU) was injected into 10-week-old male mice (G0) intraperitoneally at a dose of 250 mg/kg. The males were bred with two untreated virgin females of the same strain on day 100 after injection. The uterine contents (G1) of one female were examined on day 18.5 of pregnancy. From the other female, the offspring were delivered naturally, and F3 mice (G3) were also examined in the same way. In ICR strain mice, cleft palate only (CPO) was increased in both the G1 and G3. The frequency was significantly higher in the G3 than the G1 generation. Cleft lip was not observed. In AJ strain mice, CL/P increased in both the G1 and G3. In the G3, the frequency of CPO was increased significantly. Genes related to CPO may be recessive in phenotype. CPO and cleft lip differ from a genetic viewpoint. Point mutations play a significant role in cleft lip and palate.",
author = "Tomohiro Yamada and K. Fujiwara and K. Mishima and T. Sugahara",
year = "2005",
month = "1",
day = "1",
doi = "10.1016/j.ijom.2004.03.004",
language = "English",
volume = "34",
pages = "74--77",
journal = "International Journal of Oral and Maxillofacial Surgery",
issn = "0901-5027",
publisher = "Churchill Livingstone",
number = "1",

}

TY - JOUR

T1 - Effect of ENU (ethylnitrosourea) mutagenesis in cleft lip and/or palate pathogenesis in mice

AU - Yamada, Tomohiro

AU - Fujiwara, K.

AU - Mishima, K.

AU - Sugahara, T.

PY - 2005/1/1

Y1 - 2005/1/1

N2 - Cleft lip and/or palate (CL/P) are caused by many factors. The aim of this study was to investigate the effects of genetic point mutations in CL/P pathogenesis. ICR and AJ strain mice were used. Ethylnitrosourea (ENU) was injected into 10-week-old male mice (G0) intraperitoneally at a dose of 250 mg/kg. The males were bred with two untreated virgin females of the same strain on day 100 after injection. The uterine contents (G1) of one female were examined on day 18.5 of pregnancy. From the other female, the offspring were delivered naturally, and F3 mice (G3) were also examined in the same way. In ICR strain mice, cleft palate only (CPO) was increased in both the G1 and G3. The frequency was significantly higher in the G3 than the G1 generation. Cleft lip was not observed. In AJ strain mice, CL/P increased in both the G1 and G3. In the G3, the frequency of CPO was increased significantly. Genes related to CPO may be recessive in phenotype. CPO and cleft lip differ from a genetic viewpoint. Point mutations play a significant role in cleft lip and palate.

AB - Cleft lip and/or palate (CL/P) are caused by many factors. The aim of this study was to investigate the effects of genetic point mutations in CL/P pathogenesis. ICR and AJ strain mice were used. Ethylnitrosourea (ENU) was injected into 10-week-old male mice (G0) intraperitoneally at a dose of 250 mg/kg. The males were bred with two untreated virgin females of the same strain on day 100 after injection. The uterine contents (G1) of one female were examined on day 18.5 of pregnancy. From the other female, the offspring were delivered naturally, and F3 mice (G3) were also examined in the same way. In ICR strain mice, cleft palate only (CPO) was increased in both the G1 and G3. The frequency was significantly higher in the G3 than the G1 generation. Cleft lip was not observed. In AJ strain mice, CL/P increased in both the G1 and G3. In the G3, the frequency of CPO was increased significantly. Genes related to CPO may be recessive in phenotype. CPO and cleft lip differ from a genetic viewpoint. Point mutations play a significant role in cleft lip and palate.

UR - http://www.scopus.com/inward/record.url?scp=11844267222&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=11844267222&partnerID=8YFLogxK

U2 - 10.1016/j.ijom.2004.03.004

DO - 10.1016/j.ijom.2004.03.004

M3 - Article

C2 - 15617970

AN - SCOPUS:11844267222

VL - 34

SP - 74

EP - 77

JO - International Journal of Oral and Maxillofacial Surgery

JF - International Journal of Oral and Maxillofacial Surgery

SN - 0901-5027

IS - 1

ER -