TY - JOUR
T1 - Effect of extracellular adenosine 5'-triphosphate on principal neurons of the rat ventral tegmental area
AU - Poelchen, Wolfgang
AU - Sieler, Dirk
AU - Inoue, Kazuhide
AU - Illes, Peter
N1 - Funding Information:
We are grateful to Dr. N.B. Mercuri for showing us the preparation of midbrain slices. This work was supported by the Bundesministerium für Bildung, Forschung und Technologie, Interdisziplinäres Zentrum für Klinische Forschung an der Medizinischen Fakultät Leipzig (01KS9504; C4).
PY - 1998/7/27
Y1 - 1998/7/27
N2 - Intracellutar recordings were made in a midbrain slice preparation of the rat brain containing the ventral tegmental area (VTA). Dopaminergic principal cells were identified by their electrophysiological properties and their hyperpolarizing responses to dopamine. Superfusion with dopamine (100 μM) caused hyperpolarization and a decrease of the apparent input resistance. By contrast, two structural analogues of ATP, 2-methylthio ATP (2-MeSATP; 10 μM) and α β-methylene ATP (α, β-meATP; 30 μM) had no effect, when added to the superfusion medium. Pressure applied dopamine also hyperpolarized the membrane, while both 2-MeSATP and α, β-meATP were ineffective. Hence, dopaminergic principal neurons of the VTA do not possess somatic P2 purinoceptors present on peripheral and central noradrenergic neurons.
AB - Intracellutar recordings were made in a midbrain slice preparation of the rat brain containing the ventral tegmental area (VTA). Dopaminergic principal cells were identified by their electrophysiological properties and their hyperpolarizing responses to dopamine. Superfusion with dopamine (100 μM) caused hyperpolarization and a decrease of the apparent input resistance. By contrast, two structural analogues of ATP, 2-methylthio ATP (2-MeSATP; 10 μM) and α β-methylene ATP (α, β-meATP; 30 μM) had no effect, when added to the superfusion medium. Pressure applied dopamine also hyperpolarized the membrane, while both 2-MeSATP and α, β-meATP were ineffective. Hence, dopaminergic principal neurons of the VTA do not possess somatic P2 purinoceptors present on peripheral and central noradrenergic neurons.
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U2 - 10.1016/S0006-8993(98)00521-6
DO - 10.1016/S0006-8993(98)00521-6
M3 - Article
C2 - 9685629
AN - SCOPUS:0032572587
SN - 0006-8993
VL - 800
SP - 170
EP - 173
JO - Molecular Brain Research
JF - Molecular Brain Research
IS - 1
ER -