TY - JOUR
T1 - Effect of fluid force on vascular cell function
AU - Kudo, Susumu
AU - Yamaguchi, Ryuhei
AU - Ikeda, Mariko
AU - Tanishita, Kazuo
PY - 2005/7/1
Y1 - 2005/7/1
N2 - Endothelial cells (ECs) that line the inner surface of blood vessels are continuously exposed to fluid frictional force (shear stress) induced by blood flow, and shear stress affects the intracellular calcium ([Ca 2+] i), which initiates cellular responses. Here, we studied the effect of long-term exposure of shear stress on [Ca 2+] i responses in cultured ECs by using a confocal laser microscope and calcium indicator. At the initiation of shear stress of 20dyn/cm 2 (0 hr), 27% of the cells exhibited [Ca 2+] i responses. This percentage gradually decreased with increasing exposure time, reaching about 4% after 24 hr of exposure. These data indicate that long-term shear-stress exposure affects [Ca 2+] i responses in cultured ECs. Furthermore, we studied the effect of magnitude of shear stress on macromolecule uptake. For the low shear-stress, the uptake was enhanced, whereas the uptake was inhibited for higher shear-stress.
AB - Endothelial cells (ECs) that line the inner surface of blood vessels are continuously exposed to fluid frictional force (shear stress) induced by blood flow, and shear stress affects the intracellular calcium ([Ca 2+] i), which initiates cellular responses. Here, we studied the effect of long-term exposure of shear stress on [Ca 2+] i responses in cultured ECs by using a confocal laser microscope and calcium indicator. At the initiation of shear stress of 20dyn/cm 2 (0 hr), 27% of the cells exhibited [Ca 2+] i responses. This percentage gradually decreased with increasing exposure time, reaching about 4% after 24 hr of exposure. These data indicate that long-term shear-stress exposure affects [Ca 2+] i responses in cultured ECs. Furthermore, we studied the effect of magnitude of shear stress on macromolecule uptake. For the low shear-stress, the uptake was enhanced, whereas the uptake was inhibited for higher shear-stress.
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U2 - 10.2114/jpa.24.459
DO - 10.2114/jpa.24.459
M3 - Article
C2 - 16079597
AN - SCOPUS:26844436945
VL - 24
SP - 459
EP - 461
JO - Journal of Physiological Anthropology
JF - Journal of Physiological Anthropology
SN - 1880-6791
IS - 4
ER -