Effect of gap-junctional cell-to-cell communication on intracellular ice propagation and consequent cell viability

Takanobu Fukunaga, Hiroto Kubo, Kosaku Kurata, Hideto Hirahara, Hai Dong Wang, Hiroshi Takamatsu

研究成果: Chapter in Book/Report/Conference proceedingConference contribution

抄録

Intracellular ice formation (IIF) propagates from cell to cell and causes lethal damage to the cell during the freezing process. In this study, we hypothesized that IIF passes through gap junctions between cells and that blockage of gap junctions increases cell viability after the freeze-thaw process because of the inhibited IIF propagation. Monolayer cultures of Madin-Darby canine kidney (MDCK) cells on glass coverslips were preincubated with and without the gap-junction blocker heptanol and subsequently frozen at different cooling rates on a temperature-controlled stage. Microscopic observations showed that IIF propagation was successfully inhibited in the blocked group. However, cell viability in the blocked group after thawing was significantly lower than that in the non-blocked group. Although results of this study did not confirm our hypotheses, they indicated the important role of gap-junctional communication in the occurrence of IIF and the consequent cell damage.

本文言語英語
ホスト出版物のタイトル24th IIR International Congress of Refrigeration, ICR 2015
出版社International Institute of Refrigeration
ページ4521-4527
ページ数7
ISBN(電子版)9782362150128
DOI
出版ステータス出版済み - 1 1 2015
イベント24th IIR International Congress of Refrigeration, ICR 2015 - Yokohama, 日本
継続期間: 8 16 20158 22 2015

出版物シリーズ

名前Refrigeration Science and Technology
ISSN(印刷版)0151-1637

その他

その他24th IIR International Congress of Refrigeration, ICR 2015
国/地域日本
CityYokohama
Period8/16/158/22/15

All Science Journal Classification (ASJC) codes

  • 制御およびシステム工学
  • 電子工学および電気工学
  • 機械工学
  • 凝縮系物理学

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