Effect of gap-junctional cell-to-cell communication on intracellular ice propagation and consequent cell viability

Takanobu Fukunaga, Hiroto Kubo, Kosaku Kurata, Hideto Hirahara, Hai Dong Wang, Hiroshi Takamatsu

研究成果: 著書/レポートタイプへの貢献会議での発言

抄録

Intracellular ice formation (IIF) propagates from cell to cell and causes lethal damage to the cell during the freezing process. In this study, we hypothesized that IIF passes through gap junctions between cells and that blockage of gap junctions increases cell viability after the freeze-thaw process because of the inhibited IIF propagation. Monolayer cultures of Madin-Darby canine kidney (MDCK) cells on glass coverslips were preincubated with and without the gap-junction blocker heptanol and subsequently frozen at different cooling rates on a temperature-controlled stage. Microscopic observations showed that IIF propagation was successfully inhibited in the blocked group. However, cell viability in the blocked group after thawing was significantly lower than that in the non-blocked group. Although results of this study did not confirm our hypotheses, they indicated the important role of gap-junctional communication in the occurrence of IIF and the consequent cell damage.

元の言語英語
ホスト出版物のタイトル24th IIR International Congress of Refrigeration, ICR 2015
出版者International Institute of Refrigeration
ページ4521-4527
ページ数7
ISBN(電子版)9782362150128
DOI
出版物ステータス出版済み - 1 1 2015
イベント24th IIR International Congress of Refrigeration, ICR 2015 - Yokohama, 日本
継続期間: 8 16 20158 22 2015

出版物シリーズ

名前Refrigeration Science and Technology
ISSN(印刷物)0151-1637

その他

その他24th IIR International Congress of Refrigeration, ICR 2015
日本
Yokohama
期間8/16/158/22/15

Fingerprint

ice formation
viability
Ice
ice
communication
Cells
propagation
Communication
cells
damage
Thawing
kidneys
Freezing
freezing
Monolayers
melting
occurrences
Cooling
cooling
Glass

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering
  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Condensed Matter Physics

これを引用

Fukunaga, T., Kubo, H., Kurata, K., Hirahara, H., Wang, H. D., & Takamatsu, H. (2015). Effect of gap-junctional cell-to-cell communication on intracellular ice propagation and consequent cell viability. : 24th IIR International Congress of Refrigeration, ICR 2015 (pp. 4521-4527). (Refrigeration Science and Technology). International Institute of Refrigeration. https://doi.org/10.18462/iir.icr.2015.0437

Effect of gap-junctional cell-to-cell communication on intracellular ice propagation and consequent cell viability. / Fukunaga, Takanobu; Kubo, Hiroto; Kurata, Kosaku; Hirahara, Hideto; Wang, Hai Dong; Takamatsu, Hiroshi.

24th IIR International Congress of Refrigeration, ICR 2015. International Institute of Refrigeration, 2015. p. 4521-4527 (Refrigeration Science and Technology).

研究成果: 著書/レポートタイプへの貢献会議での発言

Fukunaga, T, Kubo, H, Kurata, K, Hirahara, H, Wang, HD & Takamatsu, H 2015, Effect of gap-junctional cell-to-cell communication on intracellular ice propagation and consequent cell viability. : 24th IIR International Congress of Refrigeration, ICR 2015. Refrigeration Science and Technology, International Institute of Refrigeration, pp. 4521-4527, 24th IIR International Congress of Refrigeration, ICR 2015, Yokohama, 日本, 8/16/15. https://doi.org/10.18462/iir.icr.2015.0437
Fukunaga T, Kubo H, Kurata K, Hirahara H, Wang HD, Takamatsu H. Effect of gap-junctional cell-to-cell communication on intracellular ice propagation and consequent cell viability. : 24th IIR International Congress of Refrigeration, ICR 2015. International Institute of Refrigeration. 2015. p. 4521-4527. (Refrigeration Science and Technology). https://doi.org/10.18462/iir.icr.2015.0437
Fukunaga, Takanobu ; Kubo, Hiroto ; Kurata, Kosaku ; Hirahara, Hideto ; Wang, Hai Dong ; Takamatsu, Hiroshi. / Effect of gap-junctional cell-to-cell communication on intracellular ice propagation and consequent cell viability. 24th IIR International Congress of Refrigeration, ICR 2015. International Institute of Refrigeration, 2015. pp. 4521-4527 (Refrigeration Science and Technology).
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