Effect of high pressure treatment on cytoplasmic 5′-nucleotidase from rabbit skeletal muscle

Sunao Mori, Atsuko Yokoyama, Reiko Iguchi, Shuhei Yamamoto, Atsushi Suzuki, Wataru Mizunoya, Ryuichi Tatsumi, Keiko Yoshioka, Yoshihide Ikeuchi

研究成果: Contribution to journalArticle査読

5 被引用数 (Scopus)

抄録

We investigated the effect of high-pressure treatment on the properties of cytoplasmic 5′-nucleotidase (NT), which converts inosine monophosphate (IMP) into inosine. After pressure treatment at 400 MPa, the activity of purified IMP-NT remained at almost 100%, but the activity of partially purified adenosine monophosphate (AMP)-NT decreased to about 40%. These data suggest that there is a difference in the pressure stability between the enzymes. In situ fluorescence spectroscopy of IMP-NT under pressure showed that its pressure-induced denaturation was reversible. When the pressure was reduced from the highest pressure to ambient pressure, hysteresis was observed. This suggests that high pressure treatment may lead to a partial change in the affinity of the subunits for each other once they have dissociated. The activities of IMP-NT and AMP-NT extracted from pressure-treated muscles decreased remarkably between 250 and 450 MPa, but IMP-NT was more stable than AMP-NT.

本文言語英語
ページ(範囲)314-327
ページ数14
ジャーナルJournal of Food Biochemistry
31
3
DOI
出版ステータス出版済み - 6 2007

All Science Journal Classification (ASJC) codes

  • Food Science
  • Biophysics
  • Pharmacology
  • Cell Biology

フィンガープリント 「Effect of high pressure treatment on cytoplasmic 5′-nucleotidase from rabbit skeletal muscle」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル