TY - JOUR
T1 - Effect of hydrothermal treatment with distilled water on titanium alloy for epithelial cellular attachment
AU - Sakamoto, Yasushige
AU - Ayukawa, Yasunori
AU - Furuhashi, Akihiro
AU - Kamo, Michimasa
AU - Ikeda, Junji
AU - Atsuta, Ikiru
AU - Haraguchi, Takuya
AU - Koyano, Kiyoshi
N1 - Funding Information:
This research was partly supported by JSPS KAKENHI, grant number JP18K17149 to T.H. from the Japan Society for the Promotion of Science. This research was also funded by KYOCERA Corporation. The authors thank the members of the Department of Biomaterials, Faculty of Dental Science, Kyushu University for technical support. The GE1 mouse-derived gingival epithelial cell line was provided by RIKEN BRC which is participating in the National BioResource Project of the MEXT/AMED, Japan.
Publisher Copyright:
© 2019 by the authors.
PY - 2019
Y1 - 2019
N2 - The enhancement of oral epithelial adhesion to the trans-mucosal material of dental implants may improve their long-term stability. The aim of this study is to investigate whether hydrothermal treatment with distilled water (HT-DW) applied to a Ti-6Al-4V (Ti64) alloy could improve epithelial cellular attachment. We hypothesized that this treatment would enhance the adsorption of proteins and the adhesion of gingival epithelial GE1 cells. This treatment changed the surface crystal structure into an anatase type of titanium oxide without an apparent change of surface roughness or topography. Nitrogen was not detected on the HT-DW-treated Ti64, which indicates decontamination. HT-DW-treated Ti64 exhibited a hydrophilic surface with a less than 10° angle of water contact. Adsorption of laminin-332 to the HT-DW-treated Ti64 was significantly greater than that of the untreated Ti64 plates (64). The number of GE1 cells on the HT-DW-treated Ti64 at 1 and 3 days was significantly lower than that on 64; however, cell adhesion strength on HT-DW was greater, with a higher expression of integrin β 4, compared with 64. This indicates that the HT-DW treatment of Ti64 improves the integration of GE1 cells, which might facilitate the development of a soft tissue barrier around the implant.
AB - The enhancement of oral epithelial adhesion to the trans-mucosal material of dental implants may improve their long-term stability. The aim of this study is to investigate whether hydrothermal treatment with distilled water (HT-DW) applied to a Ti-6Al-4V (Ti64) alloy could improve epithelial cellular attachment. We hypothesized that this treatment would enhance the adsorption of proteins and the adhesion of gingival epithelial GE1 cells. This treatment changed the surface crystal structure into an anatase type of titanium oxide without an apparent change of surface roughness or topography. Nitrogen was not detected on the HT-DW-treated Ti64, which indicates decontamination. HT-DW-treated Ti64 exhibited a hydrophilic surface with a less than 10° angle of water contact. Adsorption of laminin-332 to the HT-DW-treated Ti64 was significantly greater than that of the untreated Ti64 plates (64). The number of GE1 cells on the HT-DW-treated Ti64 at 1 and 3 days was significantly lower than that on 64; however, cell adhesion strength on HT-DW was greater, with a higher expression of integrin β 4, compared with 64. This indicates that the HT-DW treatment of Ti64 improves the integration of GE1 cells, which might facilitate the development of a soft tissue barrier around the implant.
UR - http://www.scopus.com/inward/record.url?scp=85071848683&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85071848683&partnerID=8YFLogxK
U2 - 10.3390/ma12172748
DO - 10.3390/ma12172748
M3 - Article
AN - SCOPUS:85071848683
SN - 1996-1944
VL - 12
JO - Materials
JF - Materials
IS - 7
M1 - 2748
ER -