Effect of insulin-like growth factors on lung development in a nitrofen-induced CDH rat model

Genshiro Esumi, Kouji Masumoto, Risa Teshiba, Kouji Nagata, Yoshiaki Kinoshita, Haruyoshi Yamaza, Kazuaki Nonaka, Tomoaki Taguchi

研究成果: ジャーナルへの寄稿記事

2 引用 (Scopus)

抄録

Purpose: Both the mortality and morbidity associated with congenital diaphragmatic hernia (CDH) are mainly caused by pulmonary hypoplasia and persistent pulmonary hypertension. A previous study revealed that insulin-like growth factors (IGFs) play important roles in fetal lung development. The aim of this study was to investigate the effect of IGF-1 and IGF-2 on tissue cultures of fetal hypoplastic lungs obtained from nitrofen-induced CDH model rats. Methods: Pregnant rats were exposed to nitrofen on day 9 of gestation (D9). Fetuses were harvested on D18 by caesarian section. Lung specimens of the CDH (+) fetus were divided into three groups; control, IGF-1, and IGF-2. The specimens from the control group were cultured in culture medium without IGFs. The IGF-1 group specimens were cultured with IGF-1 (500 ng/ml), and those in the IGF-2 group were cultured with IGF-2 (500 ng/ml). The mRNA expression of TTF-1, T1α and α-SMA were analyzed in each group using real-time RT-PCR after 24 and 48 h of incubation. Immunohistochemical staining of these markers was also assessed for each of the cultured specimens. Results: There was a significant increase in the expression of both TTF-1 and T1α mRNA in the IGF-2 group, in comparison to the control group after 48 h of culture. Immunohistochemical staining revealed that the cell morphology was changed from cuboidal to squamous type in the IGF-2 group. Conclusions: An increased mRNA expression of the markers related to type 1 and 2 alveolar epithelial cells, and morphological changes in the epithelial cells were observed in the IGF-2 group. The administration of IGF-2 to nitrofen-induced hypoplastic lungs might lead to alveolar maturation, which thus results in their improved development.

元の言語英語
ページ(範囲)187-192
ページ数6
ジャーナルPediatric surgery international
27
発行部数2
DOI
出版物ステータス出版済み - 2 1 2011

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Somatomedins
Lung
Fetus
Congenital Diaphragmatic Hernias
nitrofen
Messenger RNA
Staining and Labeling
Alveolar Epithelial Cells
Control Groups
Fetal Development
Pulmonary Hypertension
Cesarean Section
Culture Media
Real-Time Polymerase Chain Reaction
Epithelial Cells

All Science Journal Classification (ASJC) codes

  • Pediatrics, Perinatology, and Child Health
  • Surgery

これを引用

Effect of insulin-like growth factors on lung development in a nitrofen-induced CDH rat model. / Esumi, Genshiro; Masumoto, Kouji; Teshiba, Risa; Nagata, Kouji; Kinoshita, Yoshiaki; Yamaza, Haruyoshi; Nonaka, Kazuaki; Taguchi, Tomoaki.

:: Pediatric surgery international, 巻 27, 番号 2, 01.02.2011, p. 187-192.

研究成果: ジャーナルへの寄稿記事

Esumi, Genshiro ; Masumoto, Kouji ; Teshiba, Risa ; Nagata, Kouji ; Kinoshita, Yoshiaki ; Yamaza, Haruyoshi ; Nonaka, Kazuaki ; Taguchi, Tomoaki. / Effect of insulin-like growth factors on lung development in a nitrofen-induced CDH rat model. :: Pediatric surgery international. 2011 ; 巻 27, 番号 2. pp. 187-192.
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T1 - Effect of insulin-like growth factors on lung development in a nitrofen-induced CDH rat model

AU - Esumi, Genshiro

AU - Masumoto, Kouji

AU - Teshiba, Risa

AU - Nagata, Kouji

AU - Kinoshita, Yoshiaki

AU - Yamaza, Haruyoshi

AU - Nonaka, Kazuaki

AU - Taguchi, Tomoaki

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Y1 - 2011/2/1

N2 - Purpose: Both the mortality and morbidity associated with congenital diaphragmatic hernia (CDH) are mainly caused by pulmonary hypoplasia and persistent pulmonary hypertension. A previous study revealed that insulin-like growth factors (IGFs) play important roles in fetal lung development. The aim of this study was to investigate the effect of IGF-1 and IGF-2 on tissue cultures of fetal hypoplastic lungs obtained from nitrofen-induced CDH model rats. Methods: Pregnant rats were exposed to nitrofen on day 9 of gestation (D9). Fetuses were harvested on D18 by caesarian section. Lung specimens of the CDH (+) fetus were divided into three groups; control, IGF-1, and IGF-2. The specimens from the control group were cultured in culture medium without IGFs. The IGF-1 group specimens were cultured with IGF-1 (500 ng/ml), and those in the IGF-2 group were cultured with IGF-2 (500 ng/ml). The mRNA expression of TTF-1, T1α and α-SMA were analyzed in each group using real-time RT-PCR after 24 and 48 h of incubation. Immunohistochemical staining of these markers was also assessed for each of the cultured specimens. Results: There was a significant increase in the expression of both TTF-1 and T1α mRNA in the IGF-2 group, in comparison to the control group after 48 h of culture. Immunohistochemical staining revealed that the cell morphology was changed from cuboidal to squamous type in the IGF-2 group. Conclusions: An increased mRNA expression of the markers related to type 1 and 2 alveolar epithelial cells, and morphological changes in the epithelial cells were observed in the IGF-2 group. The administration of IGF-2 to nitrofen-induced hypoplastic lungs might lead to alveolar maturation, which thus results in their improved development.

AB - Purpose: Both the mortality and morbidity associated with congenital diaphragmatic hernia (CDH) are mainly caused by pulmonary hypoplasia and persistent pulmonary hypertension. A previous study revealed that insulin-like growth factors (IGFs) play important roles in fetal lung development. The aim of this study was to investigate the effect of IGF-1 and IGF-2 on tissue cultures of fetal hypoplastic lungs obtained from nitrofen-induced CDH model rats. Methods: Pregnant rats were exposed to nitrofen on day 9 of gestation (D9). Fetuses were harvested on D18 by caesarian section. Lung specimens of the CDH (+) fetus were divided into three groups; control, IGF-1, and IGF-2. The specimens from the control group were cultured in culture medium without IGFs. The IGF-1 group specimens were cultured with IGF-1 (500 ng/ml), and those in the IGF-2 group were cultured with IGF-2 (500 ng/ml). The mRNA expression of TTF-1, T1α and α-SMA were analyzed in each group using real-time RT-PCR after 24 and 48 h of incubation. Immunohistochemical staining of these markers was also assessed for each of the cultured specimens. Results: There was a significant increase in the expression of both TTF-1 and T1α mRNA in the IGF-2 group, in comparison to the control group after 48 h of culture. Immunohistochemical staining revealed that the cell morphology was changed from cuboidal to squamous type in the IGF-2 group. Conclusions: An increased mRNA expression of the markers related to type 1 and 2 alveolar epithelial cells, and morphological changes in the epithelial cells were observed in the IGF-2 group. The administration of IGF-2 to nitrofen-induced hypoplastic lungs might lead to alveolar maturation, which thus results in their improved development.

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