Effect of peptide content on the regulation of transgene expression by protein kinase Cα-responsive linear polyethylenimine-peptide conjugates

Riki Toita, Jeong Hun Kang, Chan Woo Kim, Shujiro Shiosaki, Takeshi Mori, Takuro Niidome, Yoshiki Katayama

研究成果: ジャーナルへの寄稿記事

3 引用 (Scopus)

抄録

We examined a series of linear polyethylenimine (LPEI)-based nanocarriers that activate transgene expression in response to cancer-specific protein kinase Cα (PKCα). Eight types of LPEI-peptide conjugate differing in peptide content and number were synthesized using click chemistry. The conjugates could form polyplexes with pDNA through electrostatic interaction, but the degree of pDNA condensation, sizes, and surface charges of the resulting polyplexes depended on the pendant-peptide content and number. None of the polyplexes showed significant cytotoxicity toward human hepatoma cells (HepG2). Furthermore, pendant peptide content and number markedly affected transgene activation in response to PKCα. To achieve an all-or-none response to PKCα, we determined the optimum peptide content and number in LPEI-peptide conjugates as ≈6. mol% and ≈40. peptides/conjugate.

元の言語英語
ページ(範囲)123-129
ページ数7
ジャーナルColloids and Surfaces B: Biointerfaces
123
DOI
出版物ステータス出版済み - 11 1 2014

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Polyethyleneimine
Transgenes
Protein Kinase C
Peptides
peptides
proteins
Proteins
Click Chemistry
Hep G2 Cells
Cytotoxicity
Surface charge
Coulomb interactions
Static Electricity
Condensation
Hepatocellular Carcinoma
condensation
cancer
Chemical activation
activation
chemistry

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

これを引用

Effect of peptide content on the regulation of transgene expression by protein kinase Cα-responsive linear polyethylenimine-peptide conjugates. / Toita, Riki; Kang, Jeong Hun; Kim, Chan Woo; Shiosaki, Shujiro; Mori, Takeshi; Niidome, Takuro; Katayama, Yoshiki.

:: Colloids and Surfaces B: Biointerfaces, 巻 123, 01.11.2014, p. 123-129.

研究成果: ジャーナルへの寄稿記事

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abstract = "We examined a series of linear polyethylenimine (LPEI)-based nanocarriers that activate transgene expression in response to cancer-specific protein kinase Cα (PKCα). Eight types of LPEI-peptide conjugate differing in peptide content and number were synthesized using click chemistry. The conjugates could form polyplexes with pDNA through electrostatic interaction, but the degree of pDNA condensation, sizes, and surface charges of the resulting polyplexes depended on the pendant-peptide content and number. None of the polyplexes showed significant cytotoxicity toward human hepatoma cells (HepG2). Furthermore, pendant peptide content and number markedly affected transgene activation in response to PKCα. To achieve an all-or-none response to PKCα, we determined the optimum peptide content and number in LPEI-peptide conjugates as ≈6. mol{\%} and ≈40. peptides/conjugate.",
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AU - Toita, Riki

AU - Kang, Jeong Hun

AU - Kim, Chan Woo

AU - Shiosaki, Shujiro

AU - Mori, Takeshi

AU - Niidome, Takuro

AU - Katayama, Yoshiki

PY - 2014/11/1

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AB - We examined a series of linear polyethylenimine (LPEI)-based nanocarriers that activate transgene expression in response to cancer-specific protein kinase Cα (PKCα). Eight types of LPEI-peptide conjugate differing in peptide content and number were synthesized using click chemistry. The conjugates could form polyplexes with pDNA through electrostatic interaction, but the degree of pDNA condensation, sizes, and surface charges of the resulting polyplexes depended on the pendant-peptide content and number. None of the polyplexes showed significant cytotoxicity toward human hepatoma cells (HepG2). Furthermore, pendant peptide content and number markedly affected transgene activation in response to PKCα. To achieve an all-or-none response to PKCα, we determined the optimum peptide content and number in LPEI-peptide conjugates as ≈6. mol% and ≈40. peptides/conjugate.

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