Effect of physical properties of nanogel particles on the kinetic constants of multipoint protein recognition process

Masahiko Nakamoto, Yu Hoshino, Yoshiko Miura

研究成果: Contribution to journalArticle査読

17 被引用数 (Scopus)

抄録

We report the effect of physical properties, such as flexibility and polymer density, of nanogel particles (NPs) on the association/dissociation rates constant (konand koff) and equilibrium constants (Kd) of multipoint protein recognition process. NPs having different flexibilities and densities at 25 °C were synthesized by tuning cross-linking degrees and the volume phase transition (VPT) temperature. Rate constants were quantified by analyzing time course of protein binding process on NPs monitored by a quartz crystal microbalance (QCM). Both konand koffof swollen phase NPs increased with decreasing cross-linking degree, whereas crosslinking degree did not affect konand k offof the collapsed phase NPs, indicating that polymer density of NPs governs konand koff. The results also suggest that the mechanical flexibility of NPs, defined as the Young's modulus, does not always have crucial roles in the multipoint molecular recognition process. On the other hand, Kdwas independent of the cross-linking degree and depended only on the phase of NPs, indicating that molecular-scale flexibility, such as side-chain and segmental-mode mobility, as well as the conformation change, of polymer chains assist the formation of stable binding sites in NPs. Our results reveal the rationale for designing NPs having desired affinity and binding kinetics to target molecules.

本文言語英語
ページ(範囲)541-547
ページ数7
ジャーナルBiomacromolecules
15
2
DOI
出版ステータス出版済み - 2 10 2014

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

フィンガープリント 「Effect of physical properties of nanogel particles on the kinetic constants of multipoint protein recognition process」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル