TY - JOUR
T1 - Effect of plasma-induced oxidative stress on the glycolysis pathway of Escherichia coli
AU - Ranjbar, S.
AU - Shahmansouri, M.
AU - Attri, P.
AU - Bogaerts, A.
N1 - Funding Information:
S. R. acknowledges funding from the Ministry of Science and Technology of Iran . The computational work was carried out using the Turing HPC infrastructure at the CalcUA core facility of the Universiteit Antwerpen (UA), a division of the Flemish Supercomputer Center VSC, funded by the Hercules Foundation , the Flemish Government (department EWI ) and the universitteit Antwerpen. We also would like to thank Dr. Charlotta Bengtson for her suggestions in writing this paper.
Publisher Copyright:
© 2020 Elsevier Ltd
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/12
Y1 - 2020/12
N2 - Antibiotic resistance is one of the world's most urgent public health problems. Due to its antibacterial properties, cold atmospheric plasma (CAP) may serve as an alternative method to antibiotics. It is claimed that oxidative stress caused by CAP is the main reason of bacteria inactivation. In this work, we computationally investigated the effect of plasma-induced oxidation on various glycolysis metabolites, by monitoring the production of the biomass. We observed that in addition to the significant reduction in biomass production, the rate of some reactions has increased. These reactions produce anti-oxidant products, showing the bacterial defense mechanism to escape the oxidative damage. Nevertheless, the simulations show that the plasma-induced oxidation effect is much stronger than the defense mechanism, causing killing of the bacteria.
AB - Antibiotic resistance is one of the world's most urgent public health problems. Due to its antibacterial properties, cold atmospheric plasma (CAP) may serve as an alternative method to antibiotics. It is claimed that oxidative stress caused by CAP is the main reason of bacteria inactivation. In this work, we computationally investigated the effect of plasma-induced oxidation on various glycolysis metabolites, by monitoring the production of the biomass. We observed that in addition to the significant reduction in biomass production, the rate of some reactions has increased. These reactions produce anti-oxidant products, showing the bacterial defense mechanism to escape the oxidative damage. Nevertheless, the simulations show that the plasma-induced oxidation effect is much stronger than the defense mechanism, causing killing of the bacteria.
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U2 - 10.1016/j.compbiomed.2020.104064
DO - 10.1016/j.compbiomed.2020.104064
M3 - Article
AN - SCOPUS:85095420692
VL - 127
JO - Computers in Biology and Medicine
JF - Computers in Biology and Medicine
SN - 0010-4825
M1 - 104064
ER -