Effect of poly(L-lactide) surface topography on the morphology of in vitro cultured human articular chondrocytes

E. Costa Martínez, J. L. Escobar Ivirico, I. Muñoz Criado, J. L. Gómez Ribelles, M. Monleón Pradas, M. Salmerón Sánchez

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抜粋

Human articular chondrocytes were cultured in vitro on poly(L-lactic) acid, PLLA, substrates. Influence of the surface topography on cell morphology was found. Different surface microtopographies were obtained on PLLA by crystallizing at 120°C after nucleation treatments that include isothermal stages at temperatures just below (55°C) and just above (75°C) the glass transition temperature (T g = 65°C). Isothermal crystallization from the melt gave rise to big spherulites (approx. 50 μm diameter) with approx. 1 μm depth. Crystallization after nucleation treatments results in smaller (approx. 5 μm)-difficult to distinguish-spherulites. Cell viability was excellent and not affected by the surface roughness. Cell population on the nucleated samples resembles the result of culture on the reference tissue culture polystyrene (TCPS). However, cells cultured on big spherulites (PLLA isothermally crystallized without nucleation treatment) show a peculiar morphology, with a more isolated disposition and growth oriented in a characteristic direction.

元の言語英語
ページ(範囲)1627-1632
ページ数6
ジャーナルJournal of Materials Science: Materials in Medicine
18
発行部数8
DOI
出版物ステータス出版済み - 8 1 2007
外部発表Yes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

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    Costa Martínez, E., Escobar Ivirico, J. L., Muñoz Criado, I., Gómez Ribelles, J. L., Monleón Pradas, M., & Salmerón Sánchez, M. (2007). Effect of poly(L-lactide) surface topography on the morphology of in vitro cultured human articular chondrocytes. Journal of Materials Science: Materials in Medicine, 18(8), 1627-1632. https://doi.org/10.1007/s10856-007-3038-1