Effect of pretreatment methods on the synergism of cellulase and xylanase during the hydrolysis of bagasse

Lili Jia, Geisa A.L. Gonçalves, Yusaku Takasugi, Yutaro Mori, Shuhei Noda, Tsutomu Tanaka, Hirofumi Ichinose, Noriho Kamiya

研究成果: ジャーナルへの寄稿記事

21 引用 (Scopus)

抄録

The effect of pretreatment with peracetic acid (PAA) or an ionic liquid (1-ethyl-3-methylimidazolium acetate, [Emim][OAc]) on the synergism between endoglucanase and endoxylanase in the hydrolysis of bagasse was investigated. An endoglucanase, Cel6A, with a carbohydrate-binding module (CBM) and two endoxylanases, XynZ-C without a CBM and Xyn11A with an intrinsic xylan/cellulose binding module (XBM), were selected. The hemicellulose content, especially arabinan, and the cellulose crystallinity of bagasse were found to affect the cellulase-xylanase synergism. More specifically, higher synergism (above 3.4) was observed for glucan conversion, at low levels of arabinan (0.9%), during the hydrolysis of PAA pretreated bagasse. In contrast, [Emim][OAc] pretreated bagasse, showed lower cellulose crystallinity and achieved higher synergism (over 1.9) for xylan conversion. Ultimately, the combination of Cel6A and Xyn11A resulted in higher synergism for glucan conversion than the combination of Cel6A with XynZ-C, indicating the importance of the molecular architecture of enzymes for metabolic synergism.

元の言語英語
ページ(範囲)158-164
ページ数7
ジャーナルBioresource Technology
185
DOI
出版物ステータス出版済み - 6 1 2015

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Bagasse
synergism
Cellulase
hydrolysis
Hydrolysis
Peracetic Acid
Cellulose
Endo-1,4-beta Xylanases
Xylans
Glucans
Carbohydrates
cellulose
crystallinity
Ionic Liquids
Acids
carbohydrate
acetate
Ionic liquids
Acetates
Enzymes

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

これを引用

Effect of pretreatment methods on the synergism of cellulase and xylanase during the hydrolysis of bagasse. / Jia, Lili; Gonçalves, Geisa A.L.; Takasugi, Yusaku; Mori, Yutaro; Noda, Shuhei; Tanaka, Tsutomu; Ichinose, Hirofumi; Kamiya, Noriho.

:: Bioresource Technology, 巻 185, 01.06.2015, p. 158-164.

研究成果: ジャーナルへの寄稿記事

Jia, Lili ; Gonçalves, Geisa A.L. ; Takasugi, Yusaku ; Mori, Yutaro ; Noda, Shuhei ; Tanaka, Tsutomu ; Ichinose, Hirofumi ; Kamiya, Noriho. / Effect of pretreatment methods on the synergism of cellulase and xylanase during the hydrolysis of bagasse. :: Bioresource Technology. 2015 ; 巻 185. pp. 158-164.
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AU - Noda, Shuhei

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