Effect of regular and irregular stimulation cycles of dexamethasone on circadian clock in NIH3T3 cells

Dan Yang, Hideaki Oike, Mitsuhiro Furuse, Shinobu Yasuo

研究成果: ジャーナルへの寄稿学術誌査読

抄録

Animal studies have shown that irregular light-dark cycles cause circadian desynchronization, while few studies have addressed the effect of regular/irregular stimulation cycles of signaling hormones on the cellular clock in vitro. Here, we examined how cellular clocks respond to regular and irregular stimulation cycles of dexamethasone, using NIH3T3 cells transfected with the Bmal1 promoter-driven luciferase (Bmal1-Luc) reporter gene. Cyclic stimulation with dexamethasone at different time intervals (18–28 h, 3 times regularly) revealed that Bmal1-Luc bioluminescence rhythms can be entrained to 22 and 24 h cycles during the stimulation period, but not to other cycles. The rhythm entrained for 24 h cycles persisted for at least one day after the last stimulation. Irregular dexamethasone treatment (16, 24, and 16 h, sequentially; short-term jet lag protocol) resulted in an overall upregulation and phase shifts of the temporal expression of several clock genes and cell cycle genes, including c-Myc and p53. Regular dexamethasone stimulation three times with 24 h cycles also caused upregulation of Per1 and Per2 expression, but not c-Myc and p53 expression. In conclusion, our study identified the entrainable range of the circadian clock in NIH3T3 cells to the dexamethasone stimulation cycle and demonstrated that irregular dexamethasone treatment could disturb the expression of cell cycle genes.

本文言語英語
ページ(範囲)97-105
ページ数9
ジャーナルChronobiology International
39
1
DOI
出版ステータス出版済み - 2022

!!!All Science Journal Classification (ASJC) codes

  • 生理学
  • 生理学(医学)

フィンガープリント

「Effect of regular and irregular stimulation cycles of dexamethasone on circadian clock in NIH3T3 cells」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル