Effect of Salithion Enantiomers on Larval Growth, Carbohydrases, Acetylcholinesterase, Adenylate Cyclase Activities and Cyclic Adenosine 3′,5′-monophosphate Level of Musca domestica and Tribolium castaneum

Akinori Hirashima, Ryohei Ueno, Kazuhiko Oyama, Hiroaki Koga, Morifusa Eto

研究成果: ジャーナルへの寄稿記事

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Dietary (R)(+)-salithion was a more potent insecticide (LC50=9.3 ppm) than the (S)(-)-enantiomer (LC50=63 ppm), correlating acetylcholinesterase (AChE) inhibition in vivo (I50=7.7 and 63 ppm) against Tribolium castaneum larvae. (S)(-)-Salioxon derived from (R)(+)-salithion had a stronger inhibitory activity (I50=8.8 μM) than the (R)(+)-enantiomer (I50=22 μM) in vitro against larval AChE. However, against Musca domestica female adults, a reversed tendency was observed: (S)(-)-salithion and (R)(+)-salioxon were more potent than (R)(+)-salithion and (S)(-)-salioxon in topical insecticidal and anticholinesterase activities in vivo and in vitro, respectively. Hence, the reversed stereospecificity between the T. castaneum larvicidal and M. domestica insecticidal activities of salithion enantiomers is due to a stereospecific difference in the intrincic potency of salioxon enantiomers as an AChE inhibitor with the two insects. A suppression of M. domestica larval growth, gut amylase and invertase activities by dietary 0.5, 1.0 and 1.25 ppm of (S)(-)-salithion was observed 2 days after treatment. A similar tendency was recognized when 1.25 and 2.50 ppm of (R)(+)-salithion was used. However, against M. domestica female adults, no appreciable effect on these enzymes was recognized 1-72 hr after topical application of 0.05 and 0.10 μg/fly of (S)(-)-salithion, and 0.01 and 0.05 μg/fly of (R)(+)-salithion. The whole-body levels of cyclic adenosine 3′, 5′-monophosphate (cAMP) were slightly increased in T. castaneum larvae 3 days after a dietary 60 and 80 ppm of (S)(-)-salithion treatment, and in M. domestica larvae 2 days after treating by dietary 1.25 and 2.50 ppm of (R)(+)-salithion, respectively. Neither of the enantiomers of salithion activated adenylate cyclase prepared from Periplaneta americana ventral nerve cords, but suppressed octopamine potency, suggesting that salithion could act as an antagonist to the octopamine-receptor and that the increased level of whole-body cAMP could be due to the inhibition of phosphodiesterase.

元の言語英語
ページ(範囲)1013-1022
ページ数10
ジャーナルAgricultural and Biological Chemistry
54
発行部数4
DOI
出版物ステータス出版済み - 1 1 1990

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Tribolium
Houseflies
adenylate cyclase
Enantiomers
Tribolium castaneum
enantiomers
cyclic AMP
Musca domestica
Acetylcholinesterase
acetylcholinesterase
Adenylyl Cyclases
Adenosine
Growth
octopamine
lethal concentration 50
larvae
ventral nerve cord
Periplaneta americana
topical application
beta-fructofuranosidase

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

これを引用

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title = "Effect of Salithion Enantiomers on Larval Growth, Carbohydrases, Acetylcholinesterase, Adenylate Cyclase Activities and Cyclic Adenosine 3′,5′-monophosphate Level of Musca domestica and Tribolium castaneum",
abstract = "Dietary (R)(+)-salithion was a more potent insecticide (LC50=9.3 ppm) than the (S)(-)-enantiomer (LC50=63 ppm), correlating acetylcholinesterase (AChE) inhibition in vivo (I50=7.7 and 63 ppm) against Tribolium castaneum larvae. (S)(-)-Salioxon derived from (R)(+)-salithion had a stronger inhibitory activity (I50=8.8 μM) than the (R)(+)-enantiomer (I50=22 μM) in vitro against larval AChE. However, against Musca domestica female adults, a reversed tendency was observed: (S)(-)-salithion and (R)(+)-salioxon were more potent than (R)(+)-salithion and (S)(-)-salioxon in topical insecticidal and anticholinesterase activities in vivo and in vitro, respectively. Hence, the reversed stereospecificity between the T. castaneum larvicidal and M. domestica insecticidal activities of salithion enantiomers is due to a stereospecific difference in the intrincic potency of salioxon enantiomers as an AChE inhibitor with the two insects. A suppression of M. domestica larval growth, gut amylase and invertase activities by dietary 0.5, 1.0 and 1.25 ppm of (S)(-)-salithion was observed 2 days after treatment. A similar tendency was recognized when 1.25 and 2.50 ppm of (R)(+)-salithion was used. However, against M. domestica female adults, no appreciable effect on these enzymes was recognized 1-72 hr after topical application of 0.05 and 0.10 μg/fly of (S)(-)-salithion, and 0.01 and 0.05 μg/fly of (R)(+)-salithion. The whole-body levels of cyclic adenosine 3′, 5′-monophosphate (cAMP) were slightly increased in T. castaneum larvae 3 days after a dietary 60 and 80 ppm of (S)(-)-salithion treatment, and in M. domestica larvae 2 days after treating by dietary 1.25 and 2.50 ppm of (R)(+)-salithion, respectively. Neither of the enantiomers of salithion activated adenylate cyclase prepared from Periplaneta americana ventral nerve cords, but suppressed octopamine potency, suggesting that salithion could act as an antagonist to the octopamine-receptor and that the increased level of whole-body cAMP could be due to the inhibition of phosphodiesterase.",
author = "Akinori Hirashima and Ryohei Ueno and Kazuhiko Oyama and Hiroaki Koga and Morifusa Eto",
year = "1990",
month = "1",
day = "1",
doi = "10.1271/bbb1961.54.1013",
language = "English",
volume = "54",
pages = "1013--1022",
journal = "Bioscience, Biotechnology and Biochemistry",
issn = "0916-8451",
publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",
number = "4",

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TY - JOUR

T1 - Effect of Salithion Enantiomers on Larval Growth, Carbohydrases, Acetylcholinesterase, Adenylate Cyclase Activities and Cyclic Adenosine 3′,5′-monophosphate Level of Musca domestica and Tribolium castaneum

AU - Hirashima, Akinori

AU - Ueno, Ryohei

AU - Oyama, Kazuhiko

AU - Koga, Hiroaki

AU - Eto, Morifusa

PY - 1990/1/1

Y1 - 1990/1/1

N2 - Dietary (R)(+)-salithion was a more potent insecticide (LC50=9.3 ppm) than the (S)(-)-enantiomer (LC50=63 ppm), correlating acetylcholinesterase (AChE) inhibition in vivo (I50=7.7 and 63 ppm) against Tribolium castaneum larvae. (S)(-)-Salioxon derived from (R)(+)-salithion had a stronger inhibitory activity (I50=8.8 μM) than the (R)(+)-enantiomer (I50=22 μM) in vitro against larval AChE. However, against Musca domestica female adults, a reversed tendency was observed: (S)(-)-salithion and (R)(+)-salioxon were more potent than (R)(+)-salithion and (S)(-)-salioxon in topical insecticidal and anticholinesterase activities in vivo and in vitro, respectively. Hence, the reversed stereospecificity between the T. castaneum larvicidal and M. domestica insecticidal activities of salithion enantiomers is due to a stereospecific difference in the intrincic potency of salioxon enantiomers as an AChE inhibitor with the two insects. A suppression of M. domestica larval growth, gut amylase and invertase activities by dietary 0.5, 1.0 and 1.25 ppm of (S)(-)-salithion was observed 2 days after treatment. A similar tendency was recognized when 1.25 and 2.50 ppm of (R)(+)-salithion was used. However, against M. domestica female adults, no appreciable effect on these enzymes was recognized 1-72 hr after topical application of 0.05 and 0.10 μg/fly of (S)(-)-salithion, and 0.01 and 0.05 μg/fly of (R)(+)-salithion. The whole-body levels of cyclic adenosine 3′, 5′-monophosphate (cAMP) were slightly increased in T. castaneum larvae 3 days after a dietary 60 and 80 ppm of (S)(-)-salithion treatment, and in M. domestica larvae 2 days after treating by dietary 1.25 and 2.50 ppm of (R)(+)-salithion, respectively. Neither of the enantiomers of salithion activated adenylate cyclase prepared from Periplaneta americana ventral nerve cords, but suppressed octopamine potency, suggesting that salithion could act as an antagonist to the octopamine-receptor and that the increased level of whole-body cAMP could be due to the inhibition of phosphodiesterase.

AB - Dietary (R)(+)-salithion was a more potent insecticide (LC50=9.3 ppm) than the (S)(-)-enantiomer (LC50=63 ppm), correlating acetylcholinesterase (AChE) inhibition in vivo (I50=7.7 and 63 ppm) against Tribolium castaneum larvae. (S)(-)-Salioxon derived from (R)(+)-salithion had a stronger inhibitory activity (I50=8.8 μM) than the (R)(+)-enantiomer (I50=22 μM) in vitro against larval AChE. However, against Musca domestica female adults, a reversed tendency was observed: (S)(-)-salithion and (R)(+)-salioxon were more potent than (R)(+)-salithion and (S)(-)-salioxon in topical insecticidal and anticholinesterase activities in vivo and in vitro, respectively. Hence, the reversed stereospecificity between the T. castaneum larvicidal and M. domestica insecticidal activities of salithion enantiomers is due to a stereospecific difference in the intrincic potency of salioxon enantiomers as an AChE inhibitor with the two insects. A suppression of M. domestica larval growth, gut amylase and invertase activities by dietary 0.5, 1.0 and 1.25 ppm of (S)(-)-salithion was observed 2 days after treatment. A similar tendency was recognized when 1.25 and 2.50 ppm of (R)(+)-salithion was used. However, against M. domestica female adults, no appreciable effect on these enzymes was recognized 1-72 hr after topical application of 0.05 and 0.10 μg/fly of (S)(-)-salithion, and 0.01 and 0.05 μg/fly of (R)(+)-salithion. The whole-body levels of cyclic adenosine 3′, 5′-monophosphate (cAMP) were slightly increased in T. castaneum larvae 3 days after a dietary 60 and 80 ppm of (S)(-)-salithion treatment, and in M. domestica larvae 2 days after treating by dietary 1.25 and 2.50 ppm of (R)(+)-salithion, respectively. Neither of the enantiomers of salithion activated adenylate cyclase prepared from Periplaneta americana ventral nerve cords, but suppressed octopamine potency, suggesting that salithion could act as an antagonist to the octopamine-receptor and that the increased level of whole-body cAMP could be due to the inhibition of phosphodiesterase.

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U2 - 10.1271/bbb1961.54.1013

DO - 10.1271/bbb1961.54.1013

M3 - Article

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SP - 1013

EP - 1022

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

SN - 0916-8451

IS - 4

ER -