Effect of salithion enantiomers on the trehalase system and on the digestive protease, amylase, and invertase of Tribolium castaneum

Akinori Hirashima, Isaac Ishaaya, Ryohei Ueno, Kazuhiko Oyama, Morifusa Eto

研究成果: ジャーナルへの寄稿記事

17 引用 (Scopus)

抄録

(R)(+)-Salithion was a more potent inhibitor than (S)(-)-enatiomer on the larval growth, pupation, and emergence of Tribolium castaneum. According to concentration needed for 50% larval weight gain inhibition (IC50), (R)(+)-enantiomer was ∼17-fold more potent than the (S)(-)-enantiomer. Larvae fed for 2 days on dietary concentration of 2 and 4 ppm (R)(+)-salithion resulted in larval weight gain inhibition of ∼10 and ∼55%, correlating well with the inhibition of the activity of the soluble larval gut trehalase. Similar correlation was obtained with 20 and 40 ppm of (S)(-)-salithion. At a concentration of 10-4 M, salithion enantiomers had no effect on the trehalase activity in vitro. Hence, the compound affects the biological process, leading to a reduced activity of trehalase in vivo. Both enantiomers had no effect in in vivo assays on the digestive protease and amylase activity. Invertase, which plays a minor role in carbohydrate digestion in Tribolium larvae, as compared to amylase, was inhibited to some extent by salithion enantiomers. Hence, the reduced activity of the trehalase system appears to be an important factor for the growth suppression observed after salithion application.

元の言語英語
ページ(範囲)205-210
ページ数6
ジャーナルPesticide Biochemistry and Physiology
34
発行部数3
DOI
出版物ステータス出版済み - 1 1 1989

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Trehalase
Tribolium
trehalase
beta-Fructofuranosidase
Digestive System
Enantiomers
Tribolium castaneum
enantiomers
Amylases
beta-fructofuranosidase
amylases
Peptide Hydrolases
proteinases
Weight Gain
weight gain
Larva
Tribolium (Coleoptera)
biochemical compounds
larvae
Biological Phenomena

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Health, Toxicology and Mutagenesis

これを引用

Effect of salithion enantiomers on the trehalase system and on the digestive protease, amylase, and invertase of Tribolium castaneum. / Hirashima, Akinori; Ishaaya, Isaac; Ueno, Ryohei; Oyama, Kazuhiko; Eto, Morifusa.

:: Pesticide Biochemistry and Physiology, 巻 34, 番号 3, 01.01.1989, p. 205-210.

研究成果: ジャーナルへの寄稿記事

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