Effect of serum depletion on centrosome overduplication and death of human pancreatic cancer cells after exposure to radiation

Masaki Shono, Norihiro Sato, Kazuhiro Mizumoto, Yohji A. Minamishima, Masafumi Nakamura, Naoki Maehara, Taro Urashima, Michiyo Saimura, Liwu Qian, Shoko Nishio, Eishi Nagai, Masao Tanaka

研究成果: ジャーナルへの寄稿学術誌査読

7 被引用数 (Scopus)

抄録

The tumor microenvironment is one of the key factors affecting the cellular response to radiation; however, the influence of serum concentration on tumor radiosensitivity remains poorly understood. We recently discovered that γ-irradiation of tumor cells causes centrosome overduplication, which may lead to lethal nuclear fragmentation through the establishment of multipolar mitotic spindles. In the present study, we investigated the effect of serum depletion on radiation-induced cell death in relation to the centrosome dynamics in human pancreatic cancer cells. Exposure of Capan-1 cells to γ-irradiation resulted in a time-dependent increase in cells containing multiple centrosomes in association with the appearance of mitotic cell death. Treatment of irradiated cells with serum depletion drastically accelerated centrosome overduplication and the formation of multipolar spindles, resulting in increased nuclear fragmentation and cell death. Cell cycle analysis of irradiated cultures revealed that the reduced serum level increased the population of cells arrested in the G2/M phase, which might be responsible for the abnormal centrosome accumulation. These findings suggest that serum concentration can influence radiation-induced cell killing through modulating cell cycle progression and possibly centrosome overduplication.

本文言語英語
ページ(範囲)81-89
ページ数9
ジャーナルCancer Letters
170
1
DOI
出版ステータス出版済み - 9月 10 2001

!!!All Science Journal Classification (ASJC) codes

  • 腫瘍学
  • 癌研究

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