Effect of the Addition of Diammonium Citrate to α-Cyano-4-Hydroxycinnamic Acid (CHCA) Matrix for the Detection of Phosphorylated Peptide in Phosphorylation Reactions Using Cell and Tissue Lysates

Jeong Hun Kang, Riki Toita, Jun Oishi, Takuro Niidome, Yoshiki Katayama

研究成果: ジャーナルへの寄稿記事

15 引用 (Scopus)

抄録

The ionization of phosphorylated peptides is usually suppressed by non-phosphorylated peptides when α-cyano-4-hydroxycinnamic acid (CHCA) is used as a matrix for matrix-assisted laser desorption/ionization-time-of-Flight (MALDI-TOF) mass spectrometry analysis. In the present study, we examined the effect of diammonium citrate addition to the CHCA matrix on the detection of phosphorylated peptides. Substrates for protein kinase C (PKC) and c-Src were synthesized and phosphorylated by reaction with cell and tissue lysate samples. The addition of diammonium citrate to the CHCA matrix increased the sensitivity for distinguishing phosphorylated peptides from background noise. However, the effect depended on substrate concentration.

元の言語英語
ページ(範囲)1925-1931
ページ数7
ジャーナルJournal of the American Society for Mass Spectrometry
18
発行部数11
DOI
出版物ステータス出版済み - 11 1 2007

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Phosphorylation
Citric Acid
Tissue
Peptides
Ionization
Substrates
Protein Kinase C
Mass spectrometry
Noise
Desorption
Mass Spectrometry
Lasers
4-coumaric acid

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Spectroscopy

これを引用

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abstract = "The ionization of phosphorylated peptides is usually suppressed by non-phosphorylated peptides when α-cyano-4-hydroxycinnamic acid (CHCA) is used as a matrix for matrix-assisted laser desorption/ionization-time-of-Flight (MALDI-TOF) mass spectrometry analysis. In the present study, we examined the effect of diammonium citrate addition to the CHCA matrix on the detection of phosphorylated peptides. Substrates for protein kinase C (PKC) and c-Src were synthesized and phosphorylated by reaction with cell and tissue lysate samples. The addition of diammonium citrate to the CHCA matrix increased the sensitivity for distinguishing phosphorylated peptides from background noise. However, the effect depended on substrate concentration.",
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T1 - Effect of the Addition of Diammonium Citrate to α-Cyano-4-Hydroxycinnamic Acid (CHCA) Matrix for the Detection of Phosphorylated Peptide in Phosphorylation Reactions Using Cell and Tissue Lysates

AU - Kang, Jeong Hun

AU - Toita, Riki

AU - Oishi, Jun

AU - Niidome, Takuro

AU - Katayama, Yoshiki

PY - 2007/11/1

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AB - The ionization of phosphorylated peptides is usually suppressed by non-phosphorylated peptides when α-cyano-4-hydroxycinnamic acid (CHCA) is used as a matrix for matrix-assisted laser desorption/ionization-time-of-Flight (MALDI-TOF) mass spectrometry analysis. In the present study, we examined the effect of diammonium citrate addition to the CHCA matrix on the detection of phosphorylated peptides. Substrates for protein kinase C (PKC) and c-Src were synthesized and phosphorylated by reaction with cell and tissue lysate samples. The addition of diammonium citrate to the CHCA matrix increased the sensitivity for distinguishing phosphorylated peptides from background noise. However, the effect depended on substrate concentration.

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