抄録
Effects of ultrasound irradiation on DNA polymerase (Klenow fragment, KF) reactions were studied on the template/primer DNA-immobilized quartz crystal microbalance (QCM). Under ultrasound irradiation, binding of KF to the DNA was suppressed due to the decrease of the binding rate constant (k1) and the increase of the dissociation rate constant (k-1). The catalytic elongation rate (kcat) was increased, but the stability of the KF/DNA/monomer ternary complex (Km) was decreased by the ultrasound irradiation. Ultrasound effects are discussed in correlation with the conformation changes of domain structures in KF.
本文言語 | 英語 |
---|---|
ページ(範囲) | 682-685 |
ページ数 | 4 |
ジャーナル | Biomacromolecules |
巻 | 7 |
号 | 3 |
DOI | |
出版ステータス | 出版済み - 3月 1 2006 |
外部発表 | はい |
All Science Journal Classification (ASJC) codes
- バイオエンジニアリング
- 生体材料
- ポリマーおよびプラスチック
- 材料化学