Effect of ultrasound on DNA polymerase reactions: Monitoring on a 27-MHz quartz crystal microbalance

Yu Hoshino, Takayoshi Kawasaki, Yoshio Okahata

研究成果: Contribution to journalArticle査読

36 被引用数 (Scopus)

抄録

Effects of ultrasound irradiation on DNA polymerase (Klenow fragment, KF) reactions were studied on the template/primer DNA-immobilized quartz crystal microbalance (QCM). Under ultrasound irradiation, binding of KF to the DNA was suppressed due to the decrease of the binding rate constant (k1) and the increase of the dissociation rate constant (k-1). The catalytic elongation rate (kcat) was increased, but the stability of the KF/DNA/monomer ternary complex (Km) was decreased by the ultrasound irradiation. Ultrasound effects are discussed in correlation with the conformation changes of domain structures in KF.

本文言語英語
ページ(範囲)682-685
ページ数4
ジャーナルBiomacromolecules
7
3
DOI
出版ステータス出版済み - 3 1 2006
外部発表はい

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

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