Effect of ultrasound on DNA polymerase reactions: Monitoring on a 27-MHz quartz crystal microbalance

Yu Hoshino; Takayoshi Kawasaki; Yoshio Okahata

doi:10.1021/bm050738e

Effect of ultrasound on DNA polymerase reactions: Monitoring on a 27-MHz quartz crystal microbalance

Yu Hoshino, Takayoshi Kawasaki, Yoshio Okahata

研究成果: ジャーナルへの寄稿 › 学術誌 › 査読

37 被引用数 (Scopus)

抄録

Effects of ultrasound irradiation on DNA polymerase (Klenow fragment, KF) reactions were studied on the template/primer DNA-immobilized quartz crystal microbalance (QCM). Under ultrasound irradiation, binding of KF to the DNA was suppressed due to the decrease of the binding rate constant (k₁) and the increase of the dissociation rate constant (k_-1). The catalytic elongation rate (k_cat) was increased, but the stability of the KF/DNA/monomer ternary complex (K_m) was decreased by the ultrasound irradiation. Ultrasound effects are discussed in correlation with the conformation changes of domain structures in KF.

本文言語	英語
ページ（範囲）	682-685
ページ数	4
ジャーナル	Biomacromolecules
巻	7
号	3
DOI	https://doi.org/10.1021/bm050738e
出版ステータス	出版済み - 3月 1 2006
外部発表	はい

All Science Journal Classification (ASJC) codes

バイオエンジニアリング
生体材料
ポリマーおよびプラスチック
材料化学

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10.1021/bm050738e

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abstract = "Effects of ultrasound irradiation on DNA polymerase (Klenow fragment, KF) reactions were studied on the template/primer DNA-immobilized quartz crystal microbalance (QCM). Under ultrasound irradiation, binding of KF to the DNA was suppressed due to the decrease of the binding rate constant (k1) and the increase of the dissociation rate constant (k-1). The catalytic elongation rate (kcat) was increased, but the stability of the KF/DNA/monomer ternary complex (Km) was decreased by the ultrasound irradiation. Ultrasound effects are discussed in correlation with the conformation changes of domain structures in KF.",

author = "Yu Hoshino and Takayoshi Kawasaki and Yoshio Okahata",

year = "2006",

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day = "1",

doi = "10.1021/bm050738e",

language = "English",

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T1 - Effect of ultrasound on DNA polymerase reactions

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AU - Hoshino, Yu

AU - Kawasaki, Takayoshi

AU - Okahata, Yoshio

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Y1 - 2006/3/1

N2 - Effects of ultrasound irradiation on DNA polymerase (Klenow fragment, KF) reactions were studied on the template/primer DNA-immobilized quartz crystal microbalance (QCM). Under ultrasound irradiation, binding of KF to the DNA was suppressed due to the decrease of the binding rate constant (k1) and the increase of the dissociation rate constant (k-1). The catalytic elongation rate (kcat) was increased, but the stability of the KF/DNA/monomer ternary complex (Km) was decreased by the ultrasound irradiation. Ultrasound effects are discussed in correlation with the conformation changes of domain structures in KF.

AB - Effects of ultrasound irradiation on DNA polymerase (Klenow fragment, KF) reactions were studied on the template/primer DNA-immobilized quartz crystal microbalance (QCM). Under ultrasound irradiation, binding of KF to the DNA was suppressed due to the decrease of the binding rate constant (k1) and the increase of the dissociation rate constant (k-1). The catalytic elongation rate (kcat) was increased, but the stability of the KF/DNA/monomer ternary complex (Km) was decreased by the ultrasound irradiation. Ultrasound effects are discussed in correlation with the conformation changes of domain structures in KF.

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JF - Biomacromolecules

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Effect of ultrasound on DNA polymerase reactions: Monitoring on a 27-MHz quartz crystal microbalance

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