Effect of ultrasound on DNA polymerase reactions: Monitoring on a 27-MHz quartz crystal microbalance

Yu Hoshino; Takayoshi Kawasaki; Yoshio Okahata

doi:10.1021/bm050738e

Effect of ultrasound on DNA polymerase reactions: Monitoring on a 27-MHz quartz crystal microbalance

Yu Hoshino, Takayoshi Kawasaki, Yoshio Okahata

研究成果: Contribution to journal › Article › 査読

36 被引用数 (Scopus)

抄録

Effects of ultrasound irradiation on DNA polymerase (Klenow fragment, KF) reactions were studied on the template/primer DNA-immobilized quartz crystal microbalance (QCM). Under ultrasound irradiation, binding of KF to the DNA was suppressed due to the decrease of the binding rate constant (k₁) and the increase of the dissociation rate constant (k_-1). The catalytic elongation rate (k_cat) was increased, but the stability of the KF/DNA/monomer ternary complex (K_m) was decreased by the ultrasound irradiation. Ultrasound effects are discussed in correlation with the conformation changes of domain structures in KF.

本文言語	英語
ページ（範囲）	682-685
ページ数	4
ジャーナル	Biomacromolecules
巻	7
号	3
DOI	https://doi.org/10.1021/bm050738e
出版ステータス	出版済み - 3 1 2006
外部発表	はい

All Science Journal Classification (ASJC) codes

Bioengineering
Biomaterials
Polymers and Plastics
Materials Chemistry

文献へのアクセス

10.1021/bm050738e

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引用スタイル

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author = "Yu Hoshino and Takayoshi Kawasaki and Yoshio Okahata",

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