Effective delivery of DNA into tumor cells and tissues by electroporation of polymer-DNA complex

Jeong Hun Kang, Riki Toita, Takuro Niidome, Yoshiki Katayama

    研究成果: Contribution to journalArticle査読

    10 被引用数 (Scopus)

    抄録

    Electroporation is a useful means for non-viral gene delivery. Here, we investigated the use of electroporation to deliver polymer-DNA complexes into living cells using a protein kinase C (PKC)α-responsive polymer. The polymer was complexed with a luciferase-encoding DNA and electroporated into B16 melanoma cells. Gene expression from polymer-DNA complexes was 3- to 5-fold higher than from naked DNA. Moreover, after introduction of the polymer-DNA complex into tissues, luciferase levels were >2-fold higher in B16 melanoma tumors than in normal skin tissue. These results suggest that the combination of our polymer and electroporation is useful for the effective delivery of DNA into tumors.

    本文言語英語
    ページ(範囲)281-288
    ページ数8
    ジャーナルCancer Letters
    265
    2
    DOI
    出版ステータス出版済み - 7 8 2008

    All Science Journal Classification (ASJC) codes

    • 腫瘍学
    • 癌研究

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