Effective method for discriminating between oral bacterial and human alkaline phosphatase activity

Yukie Shibata, Yoshihisa Yamashita, H. Miyazaki, S. Ueno, T. Takehara

研究成果: ジャーナルへの寄稿記事

5 引用 (Scopus)

抄録

Alkaline phosphatase (ALPase) activity was quantitatively compared in various kinds of oral bacteria. High ALPase activity was detected in 3 species of periodontal bacteria, Porphyromonas gingivalis. Prevotella intermedia and Capno‐cvtophaga sputigena. The ALPase activity detected in these bacteria was almost completely inhibited in the presence of 1% sodium dodecyl sulfate (SDS). By contrast, the activity of mammalian ALPase isoenzymes was not inhibited at all even in the presence of 1% SDS. These results indicate that the ALPase assay in combination with 1% SDS can identify the origin of ALPase detected in gingival crevicular fluid as being from bacteria or from a host response. Clinical examination with adult periodontitis revealed that ALPase activity in gingival crevicular fluid from the patients consisted of a combination of SDS‐sensitive and SDS‐resistant activities. These findings indicate that ALPase activity detected in gingival crevicular fluid originates not only from bacteria but also from a host response.

元の言語英語
ページ(範囲)35-39
ページ数5
ジャーナルOral Microbiology and Immunology
9
発行部数1
DOI
出版物ステータス出版済み - 1 1 1994
外部発表Yes

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Alkaline Phosphatase
Gingival Crevicular Fluid
Bacteria
Sodium Dodecyl Sulfate
Prevotella intermedia
Chronic Periodontitis
Porphyromonas gingivalis
Isoenzymes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Dentistry(all)
  • Microbiology (medical)

これを引用

Effective method for discriminating between oral bacterial and human alkaline phosphatase activity. / Shibata, Yukie; Yamashita, Yoshihisa; Miyazaki, H.; Ueno, S.; Takehara, T.

:: Oral Microbiology and Immunology, 巻 9, 番号 1, 01.01.1994, p. 35-39.

研究成果: ジャーナルへの寄稿記事

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abstract = "Alkaline phosphatase (ALPase) activity was quantitatively compared in various kinds of oral bacteria. High ALPase activity was detected in 3 species of periodontal bacteria, Porphyromonas gingivalis. Prevotella intermedia and Capno‐cvtophaga sputigena. The ALPase activity detected in these bacteria was almost completely inhibited in the presence of 1{\%} sodium dodecyl sulfate (SDS). By contrast, the activity of mammalian ALPase isoenzymes was not inhibited at all even in the presence of 1{\%} SDS. These results indicate that the ALPase assay in combination with 1{\%} SDS can identify the origin of ALPase detected in gingival crevicular fluid as being from bacteria or from a host response. Clinical examination with adult periodontitis revealed that ALPase activity in gingival crevicular fluid from the patients consisted of a combination of SDS‐sensitive and SDS‐resistant activities. These findings indicate that ALPase activity detected in gingival crevicular fluid originates not only from bacteria but also from a host response.",
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AU - Yamashita, Yoshihisa

AU - Miyazaki, H.

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AU - Takehara, T.

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N2 - Alkaline phosphatase (ALPase) activity was quantitatively compared in various kinds of oral bacteria. High ALPase activity was detected in 3 species of periodontal bacteria, Porphyromonas gingivalis. Prevotella intermedia and Capno‐cvtophaga sputigena. The ALPase activity detected in these bacteria was almost completely inhibited in the presence of 1% sodium dodecyl sulfate (SDS). By contrast, the activity of mammalian ALPase isoenzymes was not inhibited at all even in the presence of 1% SDS. These results indicate that the ALPase assay in combination with 1% SDS can identify the origin of ALPase detected in gingival crevicular fluid as being from bacteria or from a host response. Clinical examination with adult periodontitis revealed that ALPase activity in gingival crevicular fluid from the patients consisted of a combination of SDS‐sensitive and SDS‐resistant activities. These findings indicate that ALPase activity detected in gingival crevicular fluid originates not only from bacteria but also from a host response.

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