Effective renaturation of met-1 lysozyme expressed in Escherichia Coli as inclusion bodies

Yoshitake Maeda, Tadashi Ueda, Taiji Imoto

研究成果: Contribution to journalArticle査読

1 被引用数 (Scopus)

抄録

The effective renaturation method that we developed was applied to the renaturation of the reduced Met-1 lysozyme expressed in E. coli as inclusion bodies. Using a slow dialysis renaturation method, a concentration as high as 1.25 mg/ml of the reduced Met-1 lysozyme could be renatured in 40% yield, while the folding yield was less than 5% even at a concentration of 0.25 mg/ml using a rapid dilution method.

本文言語英語
ページ(範囲)67-74
ページ数8
ジャーナルProtein and Peptide Letters
5
2
出版ステータス出版済み - 12 1 1998

All Science Journal Classification (ASJC) codes

  • 構造生物学
  • 生化学

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