Effective separation of peptides using highly dense thermo-responsive polymer brush-grafted porous polystyrene beads

Aya Mizutani, Kenichi Nagase, Akihiko Kikuchi, Hideko Kanazawa, Yoshikatsu Akiyama, Jun Kobayashi, Annaka Masahiko, Teruo Okano

研究成果: ジャーナルへの寄稿記事

25 引用 (Scopus)

抄録

For the development of well-defined highly dense thermo-responsive polymer grafted surface as an improved stationary phase for thermo-responsive chromatography, poly(N-isopropylacrylamide) (PIPAAm) brush-grafted porous polystyrene beads were prepared by surface-initiated atom transfer radical polymerization (ATRP). The PIPAAm grafted region of polystyrene beads was adjusted by the addition of isooctane as a poor solvent for polystyrene upon the reaction of ATRP initiator immobilization. Using a thermo-responsive HPLC column containing the prepared beads with PIPAAm brush grafted on the inside pores nearby the outer surfaces, angiotensin subtypes were effectively separated with aqueous mobile phase, because the densely grafted PIPAAm on nearby the outer surface effectively interacted with the peptides hydrophobically. Retention of basic peptide was achieved by the beads with basic mobile phase. These results indicated that the prepared beads with grafted PIPAAm nearby the outer surface became an effective chromatographic stationary phase for retaining basic peptides using wide pH range of mobile phase.

元の言語英語
ページ(範囲)2191-2198
ページ数8
ジャーナルJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
878
発行部数24
DOI
出版物ステータス出版済み - 8 1 2010

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Polystyrenes
Brushes
Polymers
Peptides
Atom transfer radical polymerization
Polymerization
Angiotensins
Chromatography
Immobilization
High Pressure Liquid Chromatography
poly-N-isopropylacrylamide

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

これを引用

Effective separation of peptides using highly dense thermo-responsive polymer brush-grafted porous polystyrene beads. / Mizutani, Aya; Nagase, Kenichi; Kikuchi, Akihiko; Kanazawa, Hideko; Akiyama, Yoshikatsu; Kobayashi, Jun; Masahiko, Annaka; Okano, Teruo.

:: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 巻 878, 番号 24, 01.08.2010, p. 2191-2198.

研究成果: ジャーナルへの寄稿記事

Mizutani, Aya ; Nagase, Kenichi ; Kikuchi, Akihiko ; Kanazawa, Hideko ; Akiyama, Yoshikatsu ; Kobayashi, Jun ; Masahiko, Annaka ; Okano, Teruo. / Effective separation of peptides using highly dense thermo-responsive polymer brush-grafted porous polystyrene beads. :: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2010 ; 巻 878, 番号 24. pp. 2191-2198.
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AU - Mizutani, Aya

AU - Nagase, Kenichi

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AU - Kanazawa, Hideko

AU - Akiyama, Yoshikatsu

AU - Kobayashi, Jun

AU - Masahiko, Annaka

AU - Okano, Teruo

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AB - For the development of well-defined highly dense thermo-responsive polymer grafted surface as an improved stationary phase for thermo-responsive chromatography, poly(N-isopropylacrylamide) (PIPAAm) brush-grafted porous polystyrene beads were prepared by surface-initiated atom transfer radical polymerization (ATRP). The PIPAAm grafted region of polystyrene beads was adjusted by the addition of isooctane as a poor solvent for polystyrene upon the reaction of ATRP initiator immobilization. Using a thermo-responsive HPLC column containing the prepared beads with PIPAAm brush grafted on the inside pores nearby the outer surfaces, angiotensin subtypes were effectively separated with aqueous mobile phase, because the densely grafted PIPAAm on nearby the outer surface effectively interacted with the peptides hydrophobically. Retention of basic peptide was achieved by the beads with basic mobile phase. These results indicated that the prepared beads with grafted PIPAAm nearby the outer surface became an effective chromatographic stationary phase for retaining basic peptides using wide pH range of mobile phase.

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