Two copolymers are designed and synthesised for siRNA delivery based on polyethylenimine by grafting hydrophilic acrylamide segments and hydrophobic poly(γ-benzyl L-glutamate). The amphiphilic PEI-PBLG/siRNA complex demonstrates high gene silencing efficiency in the absence or presence of 10vol% and 50vol% sera in vitro. The anti-tumor effects in vivo are evaluated in luciferase-bearing mice expressing CT26 tumors. PEI-PBLG/siVEGF complex provides a higher and more sustained suppressive effect by reducing VEGF mRNA expression in the tumors, leading to higher tumor growth inhibition efficacy. Further studies on the potential use of the PEI-PBLG carrier system in mediating the silencing of genes other than VEGF or in other tumor models are recommended. The amphiphilic copolymer conjugate PEI-PBLG can efficiently deliver siVEGF to tumor tissues and realize the downregulation of VEGF gene expression. The PEI-PBLG/siVEGF complex successfully exhibits an anti-angiogenic effect for the treatment of tumor growth inhibition in vivo.
|出版ステータス||出版済み - 10月 2013|
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