Effectiveness of vitamin A acetate for enhancing the production of lung cancer specific monoclonal antibodies

Yuichi Inoue, Mihoko Fujisawa, Seiji Kawamoto, Masahiro Shoji, Shuichi Hashizume, Makoto Fujii, Yoshinori Katakura, Sanetaka Shirahata

研究成果: Contribution to journalArticle査読

8 被引用数 (Scopus)

抄録

The antibody productivity of the human-human hybridoma cell line AE6, which produces the tung cancer specific human monoclonal antibody AE6F4, was enhanced fourfold upon stimulation with 1 μg/ml of vitamin A acetate for one day. The enhancement lasted for about two weeks, and could be repeated by another stimulation with vitamin A acetate. The enhancing effect of vitamin A acetate was influenced by the cell density. Enhancement was clearly observed when the cell density was under 106 cells/ml. However, when the cell density was over 107 cells/ml, enhancement was observed weakly or not at all. Although the enhancing effect of vitamin A acetate is not unique to AE6 cells, not all human-human hybridoma cell lines show increased productivity upon VA acetate stimulation. This study suggests that the response to vitamin A acetate may be related to the properties of a particular fusion partner which the hybridoma cell inherits. The efficacy of vitamin A acetate for production of human monoclonal antibodies using human-human hybridomas is discussed.

本文言語英語
ページ(範囲)77-83
ページ数7
ジャーナルCytotechnology
31
1-2
DOI
出版ステータス出版済み - 1999

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • バイオエンジニアリング
  • 生体医工学
  • 臨床生化学
  • 細胞生物学

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