Effects of an anti-platelet drug on the prevention of steroid-induced osteonecrosis in rabbits

Ryosuke Yamaguchi, Takuaki Yamamoto, goro motomura, Satoshi Ikemura, Kenyu Iwasaki, Garida Zhao, Yukihide Iwamoto

研究成果: ジャーナルへの寄稿記事

13 引用 (Scopus)

抄録

Objective: To investigate the effects of an anti-platelet drug (clopidogrel) on the prevention of steroid-induced osteonecrosis (ON) in rabbits. Methods: Adult male Japanese white rabbits were divided into two groups and treated as follows: one group received daily clopidogrel mixed in normal saline (AP; n = 35), the other received only normal saline (NS; n = 30). One week after the administration, all rabbits were injected once intramuscularly with 20 mg/kg of methylprednisolone acetate into the right gluteus medius muscle. Three weeks after, both the femora and humeri were examined histopathologically for the presence of ON. The platelet aggregation assay and hematological examinations were performed before and after the steroid injection. Results: The incidence of ON in the AP group (48.5%) was significantly lower than that observed in the NS group (73.3%). The platelet aggregations in the AP group were significantly inhibited by the administration of clopidogrel. The levels of total cholesterol and triglycerides showed no significant differences between the AP and NS group. Conclusion: The present experimental study demonstrated that the administration of an anti-platelet drug prevented steroid-induced ON in rabbits and that platelet aggregation seems to be one of the possible factors involved in the pathogenesis of steroid-induced ON.

元の言語英語
記事番号ker197
ページ(範囲)789-793
ページ数5
ジャーナルRheumatology
51
発行部数5
DOI
出版物ステータス出版済み - 5 1 2012

Fingerprint

clopidogrel
Osteonecrosis
Blood Platelets
Steroids
Rabbits
Platelet Aggregation
Pharmaceutical Preparations
Humerus
Femur
Triglycerides
Cholesterol
Muscles
Injections
Incidence

All Science Journal Classification (ASJC) codes

  • Rheumatology
  • Pharmacology (medical)

これを引用

Effects of an anti-platelet drug on the prevention of steroid-induced osteonecrosis in rabbits. / Yamaguchi, Ryosuke; Yamamoto, Takuaki; motomura, goro; Ikemura, Satoshi; Iwasaki, Kenyu; Zhao, Garida; Iwamoto, Yukihide.

:: Rheumatology, 巻 51, 番号 5, ker197, 01.05.2012, p. 789-793.

研究成果: ジャーナルへの寄稿記事

Yamaguchi, Ryosuke ; Yamamoto, Takuaki ; motomura, goro ; Ikemura, Satoshi ; Iwasaki, Kenyu ; Zhao, Garida ; Iwamoto, Yukihide. / Effects of an anti-platelet drug on the prevention of steroid-induced osteonecrosis in rabbits. :: Rheumatology. 2012 ; 巻 51, 番号 5. pp. 789-793.
@article{9cfa4697605149b09aa1b58e7cac546c,
title = "Effects of an anti-platelet drug on the prevention of steroid-induced osteonecrosis in rabbits",
abstract = "Objective: To investigate the effects of an anti-platelet drug (clopidogrel) on the prevention of steroid-induced osteonecrosis (ON) in rabbits. Methods: Adult male Japanese white rabbits were divided into two groups and treated as follows: one group received daily clopidogrel mixed in normal saline (AP; n = 35), the other received only normal saline (NS; n = 30). One week after the administration, all rabbits were injected once intramuscularly with 20 mg/kg of methylprednisolone acetate into the right gluteus medius muscle. Three weeks after, both the femora and humeri were examined histopathologically for the presence of ON. The platelet aggregation assay and hematological examinations were performed before and after the steroid injection. Results: The incidence of ON in the AP group (48.5{\%}) was significantly lower than that observed in the NS group (73.3{\%}). The platelet aggregations in the AP group were significantly inhibited by the administration of clopidogrel. The levels of total cholesterol and triglycerides showed no significant differences between the AP and NS group. Conclusion: The present experimental study demonstrated that the administration of an anti-platelet drug prevented steroid-induced ON in rabbits and that platelet aggregation seems to be one of the possible factors involved in the pathogenesis of steroid-induced ON.",
author = "Ryosuke Yamaguchi and Takuaki Yamamoto and goro motomura and Satoshi Ikemura and Kenyu Iwasaki and Garida Zhao and Yukihide Iwamoto",
year = "2012",
month = "5",
day = "1",
doi = "10.1093/rheumatology/ker197",
language = "English",
volume = "51",
pages = "789--793",
journal = "Rheumatology",
issn = "1462-0324",
publisher = "Oxford University Press",
number = "5",

}

TY - JOUR

T1 - Effects of an anti-platelet drug on the prevention of steroid-induced osteonecrosis in rabbits

AU - Yamaguchi, Ryosuke

AU - Yamamoto, Takuaki

AU - motomura, goro

AU - Ikemura, Satoshi

AU - Iwasaki, Kenyu

AU - Zhao, Garida

AU - Iwamoto, Yukihide

PY - 2012/5/1

Y1 - 2012/5/1

N2 - Objective: To investigate the effects of an anti-platelet drug (clopidogrel) on the prevention of steroid-induced osteonecrosis (ON) in rabbits. Methods: Adult male Japanese white rabbits were divided into two groups and treated as follows: one group received daily clopidogrel mixed in normal saline (AP; n = 35), the other received only normal saline (NS; n = 30). One week after the administration, all rabbits were injected once intramuscularly with 20 mg/kg of methylprednisolone acetate into the right gluteus medius muscle. Three weeks after, both the femora and humeri were examined histopathologically for the presence of ON. The platelet aggregation assay and hematological examinations were performed before and after the steroid injection. Results: The incidence of ON in the AP group (48.5%) was significantly lower than that observed in the NS group (73.3%). The platelet aggregations in the AP group were significantly inhibited by the administration of clopidogrel. The levels of total cholesterol and triglycerides showed no significant differences between the AP and NS group. Conclusion: The present experimental study demonstrated that the administration of an anti-platelet drug prevented steroid-induced ON in rabbits and that platelet aggregation seems to be one of the possible factors involved in the pathogenesis of steroid-induced ON.

AB - Objective: To investigate the effects of an anti-platelet drug (clopidogrel) on the prevention of steroid-induced osteonecrosis (ON) in rabbits. Methods: Adult male Japanese white rabbits were divided into two groups and treated as follows: one group received daily clopidogrel mixed in normal saline (AP; n = 35), the other received only normal saline (NS; n = 30). One week after the administration, all rabbits were injected once intramuscularly with 20 mg/kg of methylprednisolone acetate into the right gluteus medius muscle. Three weeks after, both the femora and humeri were examined histopathologically for the presence of ON. The platelet aggregation assay and hematological examinations were performed before and after the steroid injection. Results: The incidence of ON in the AP group (48.5%) was significantly lower than that observed in the NS group (73.3%). The platelet aggregations in the AP group were significantly inhibited by the administration of clopidogrel. The levels of total cholesterol and triglycerides showed no significant differences between the AP and NS group. Conclusion: The present experimental study demonstrated that the administration of an anti-platelet drug prevented steroid-induced ON in rabbits and that platelet aggregation seems to be one of the possible factors involved in the pathogenesis of steroid-induced ON.

UR - http://www.scopus.com/inward/record.url?scp=84860257944&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84860257944&partnerID=8YFLogxK

U2 - 10.1093/rheumatology/ker197

DO - 10.1093/rheumatology/ker197

M3 - Article

VL - 51

SP - 789

EP - 793

JO - Rheumatology

JF - Rheumatology

SN - 1462-0324

IS - 5

M1 - ker197

ER -