Effects of both vitamin C and mechanical stimulation on improving the mechanical characteristics of regenerated cartilage

Seiji Omata, Shogo Sonokawa, Yoshinori Sawae, Teruo Murakami

    研究成果: Contribution to journalArticle査読

    13 被引用数 (Scopus)

    抄録

    The present work describes the influence of both vitamin C (VC) and mechanical stimulation on development of the extracellular matrix (ECM) and improvement in mechanical properties of a chondrocyte-agarose construct in a regenerating tissue disease model of hyaline cartilage. We used primary bovine chondrocytes and two types of VC, ascorbic acid (AsA) as an acidic form and ascorbic acid 2-phosphate (A2P) as a non-acidic form, and applied uniaxial compressive strain to the tissue model using a purpose-built bioreactor. When added to the medium in free-swelling culture conditions, A2P downregulated development of ECM and suppressed improvement of the tangent modulus more than AsA. By contrast, application of mechanical stimulation to the construct both increased the tangent modulus more than the free-swelling group containing A2P and enhanced the ECM network of inner tissue to levels nearly as high as the free-swelling group containing AsA. Thus, mechanical stimulation and strain appears to enhance the supply of nutrients and improve the synthesis of ECM via mechanotransduction pathways of chondrocytes. Therefore, we suggest that mechanical stimulation is necessary for homogenous development of ECM in a cell-associated construct with a view to implantation of a large-sized articular cartilage defect.

    本文言語英語
    ページ(範囲)724-729
    ページ数6
    ジャーナルBiochemical and Biophysical Research Communications
    424
    4
    DOI
    出版ステータス出版済み - 8 10 2012

    All Science Journal Classification (ASJC) codes

    • 生物理学
    • 生化学
    • 分子生物学
    • 細胞生物学

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