TY - JOUR
T1 - Effects of EMD liquid (Osteogain) on periodontal healing in class III furcation defects in monkeys
AU - Shirakata, Yoshinori
AU - Miron, Richard J.
AU - Nakamura, Toshiaki
AU - Sena, Kotaro
AU - Shinohara, Yukiya
AU - Horai, Naoto
AU - Bosshardt, Dieter D.
AU - Noguchi, Kazuyuki
AU - Sculean, Anton
N1 - Funding Information:
Conflict of interest and source of funding statement The authors report no conflicts of interest related to this study. This study was partly supported by a Grant-in-Aid for Scientific Research C (No. 25463052 to Dr. Y. Shirakata) and a Grant-in-Aid for Scientific Research B (No. 15H05036 to Dr. K. Noguchi) from Japan Society for the Promotion of Science. The authors declare no conflict of interests regarding the materials tested in this study. The materials were kindly provided by Straumann, Basel Switzerland, and Botiss, Berlin Germany. The authors wish to thank Mr. Makoto Tanoue for animal technical support; Mr. Shinya Maeda, Mr. Keisuke Ishimaru, and Ms. Maya Iwase for the histological preparation of the specimens; Shin Nippon Biomedical Laboratories, Ltd, Kagoshima, Japan. Institut Straumann AG, (Basel, Switzerland) provided the Osteogain and Emdogain used in this study. The authors thank Dr. Stefano Tugulu and Dr. Benjamin E. Pippenger (Institut Straumann AG, Basel, Switzerland) for their help and scientific comments in conducting this study. Absorbable collagen sponge (Collacone®) was provided free of charge by Botiss Dental, Berlin, Germany.
Publisher Copyright:
© 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
PY - 2017/3/1
Y1 - 2017/3/1
N2 - Aim: To evaluate the effect of a novel liquid carrier system of enamel matrix derivative (Osteogain) soaked on an absorbable collagen sponge (ACS) upon periodontal wound healing/regeneration in furcation defects in monkeys. Materials and Methods: The stability of the conventional enamel matrix derivative (Emdogain) and Osteogain adsorbed onto ACS was evaluated by ELISA. Chronic class III furcation defects were created at teeth 36, 37, 46, 47 in three monkeys (Macaca fascicularis). The 12 defects were assigned to one of the following treatments: (1) open flap debridement (OFD) + ACS, (2) OFD+Emdogain/ACS, (3) OFD+Osteogain/ACS, and (4) OFD alone. At 16 weeks following reconstructive surgery, the animals were killed for histological evaluation. Results: A 20–60% significantly higher amount of total adsorbed amelogenin was found for ACS-loaded Osteogain when compared to Emdogain. The histomorphometric analysis revealed that both approaches (OFD + Emdogain/ACS and OFD + Osteogain/ACS) resulted in higher amounts of connective tissue attachment and bone formation compared to treatment with OFD + ACS and OFD alone. Furthermore, OFD + Osteogain/ACS group showed higher new attachment formation, cementum, and new bone area. Conclusions: Within their limits, the present data indicate that Osteogain possesses favourable physicochemical properties facilitating adsorption of amelogenin on ACS and may additionally enhance periodontal wound healing/regeneration when compared to Emdogain.
AB - Aim: To evaluate the effect of a novel liquid carrier system of enamel matrix derivative (Osteogain) soaked on an absorbable collagen sponge (ACS) upon periodontal wound healing/regeneration in furcation defects in monkeys. Materials and Methods: The stability of the conventional enamel matrix derivative (Emdogain) and Osteogain adsorbed onto ACS was evaluated by ELISA. Chronic class III furcation defects were created at teeth 36, 37, 46, 47 in three monkeys (Macaca fascicularis). The 12 defects were assigned to one of the following treatments: (1) open flap debridement (OFD) + ACS, (2) OFD+Emdogain/ACS, (3) OFD+Osteogain/ACS, and (4) OFD alone. At 16 weeks following reconstructive surgery, the animals were killed for histological evaluation. Results: A 20–60% significantly higher amount of total adsorbed amelogenin was found for ACS-loaded Osteogain when compared to Emdogain. The histomorphometric analysis revealed that both approaches (OFD + Emdogain/ACS and OFD + Osteogain/ACS) resulted in higher amounts of connective tissue attachment and bone formation compared to treatment with OFD + ACS and OFD alone. Furthermore, OFD + Osteogain/ACS group showed higher new attachment formation, cementum, and new bone area. Conclusions: Within their limits, the present data indicate that Osteogain possesses favourable physicochemical properties facilitating adsorption of amelogenin on ACS and may additionally enhance periodontal wound healing/regeneration when compared to Emdogain.
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U2 - 10.1111/jcpe.12663
DO - 10.1111/jcpe.12663
M3 - Article
C2 - 27978604
AN - SCOPUS:85013191386
VL - 44
SP - 298
EP - 307
JO - Journal of Clinical Periodontology
JF - Journal of Clinical Periodontology
SN - 0303-6979
IS - 3
ER -