Effects of endoglycoceramidase or D-threo-1-phenyl-2-decanoylamino-3- morpholino-1-propanol on glucose uptake, glycolysis, and mitochondrial respiration in HL60 cells

Li Ji, Makoto Ito, Gu Zhang, Yoshio Hirabayashi, Jin Ichi Inokuchi, Tatsuya Yamagata

研究成果: Contribution to journalArticle

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The glycosphingolipid content of HL60 cells was reduced by endoglycoceramidase, an enzyme which specifically hydrolyzes glycosphingolipids on the cell surface, or by D-threo-1-phenyl-2- decanoylamino-3-morpholino-1-propanol, an inhibitor which specifically reduces the activity of UDP-glucose:ceramide glucosyltransferase. Reduction of the glycosphingolipid content by both reagents resulted in enhancement of glucose uptake and glycolysis. Neither of these effects was observed in the presence of cytochalasin B, an inhibitor of facilitated glucose transport. The uptake of radiolabeled 3-O-methylglucose by the cells was not affected by treatment with either of the reagents, indicating no activation of the glucose transporter. On the other hand, both reagents decreased the level of ATP and CO2 production. The molecule mediating these effects appeared to be ceramide, since both treatments actually increased the intracellular ceramide content, and the cell-permeable short-chain ceramide N-acetylsphingosine, but not sphingosine, sphinganine, or palmitic acid, mimicked the effects of both reagents to comparable extents. Finally, the function of electron transport in isolated mitochondria fractions was found to be reduced by treatment of the cells with N-acetylsphingosine. These results strongly suggest that ceramide may affect mitochondrial respiration.

元の言語英語
ページ(範囲)107-114
ページ数8
ジャーナルArchives of Biochemistry and Biophysics
359
発行部数1
DOI
出版物ステータス出版済み - 11 1 1998

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology

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