Effects of hormones and osmolarity in the culture medium on germinal vesicle breakdown of porcine oocytes

N. Yamauchi, H. Sasada, E. Soloy, T. Dominko, K. Kikuchi, T. Nagai

研究成果: Contribution to journalArticle査読

10 被引用数 (Scopus)

抄録

The present study was conducted to examine effects of hormones and osmolarity on germinal vesicle breakdown (GVBD) and histone H1 kinase (H1K) activity in porcine oocytes cultured in vitro. The basic medium used for culture of oocytes was modified Tyrode's solution in which the osmolarity was adjusted to 134 to 495 mOsm by changing the concentration of sodium chloride (NaCl). When the hormones were present, osmolarity of medium that allows GVBD of oocytes was less than 400 mOsm. However, the range of osmolarity of medium that allows meiotic maturation of oocytes was 210 to 362 mOsm. On the other hand, without hormonal supplement, the incidence of GVBD in oocytes decreased as the osmolarity of the medium increased in the rage of 210 to 362 mOsm. By increasing the osmolarity of the medium from 210 to 362 mOsm by addition with sorbitol instead of NaCl, the incidence decreased from 89.1% to 13.3%. In oocytes cultured in medium of 210 mOsm without hormones, the percentage of oocytes that underwent GVBD and had increased H1K activity 20 h after culture was significantly higher (P<0.05) than those of oocytes cultured in the same medium supplemented with hormones or medium of 362 mOsm. These results indicate that in vitro induction of GVBD in porcine oocytes is strongly affected by osmolarity of the medium in the absence of hormones. The results also suggest that under low osmolarity (210 mOsm), GVBD is accelerated with rapid increase of H1K activity.

本文言語英語
ページ(範囲)153-162
ページ数10
ジャーナルTheriogenology
52
1
DOI
出版ステータス出版済み - 7 1 1999
外部発表はい

All Science Journal Classification (ASJC) codes

  • Small Animals
  • Food Animals
  • Animal Science and Zoology
  • Equine

フィンガープリント 「Effects of hormones and osmolarity in the culture medium on germinal vesicle breakdown of porcine oocytes」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル