Effects of Host Cell Density on Cell Infection Level in Antheraea eucalypti (Lepidoptera: Saturniidae) Cell Cultures Persistently Infected with Nosema bombycis (Microsporida: Nosematidae)

Chisa Yasunaga-Aoki, MASAKO FUNAKOSHI, TAKESHI KAWARABATA

研究成果: ジャーナルへの寄稿記事

4 引用 (Scopus)

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ABSTRACT. Spores of Nosema bombycis Y9101, isolated from the beet armyworm, Spodoptera exigua, were primed with an alkaline solution and inoculated into Antheraea eucalypti cell cultures. Infected cells were subcultured every five days at three cell densities (2.5 × 103, 5.0 × 103, and 1.0 × 104 cells/cm2). A difference was observed in the spread of N. bombycis Y9101 infection between low‐density and higher‐density cultures of host cells. The host cell density did not affect the productivity of secondary infective forms of the parasite. The principal factor determining the rate of microsporidian infection in vitro was the number of host cells existing within the reach of extruded short‐coiled polar tubes from spores germinated intracellularly.

元の言語英語
ページ(範囲)133-137
ページ数5
ジャーナルJournal of Eukaryotic Microbiology
41
発行部数2
DOI
出版物ステータス出版済み - 1 1 1994

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Microsporida
Nosema
Eucalyptus
Lepidoptera
Moths
Cell Culture Techniques
Cell Count
Spores
Infection
Spodoptera
Beta vulgaris
Parasites

All Science Journal Classification (ASJC) codes

  • Microbiology

これを引用

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title = "Effects of Host Cell Density on Cell Infection Level in Antheraea eucalypti (Lepidoptera: Saturniidae) Cell Cultures Persistently Infected with Nosema bombycis (Microsporida: Nosematidae)",
abstract = "ABSTRACT. Spores of Nosema bombycis Y9101, isolated from the beet armyworm, Spodoptera exigua, were primed with an alkaline solution and inoculated into Antheraea eucalypti cell cultures. Infected cells were subcultured every five days at three cell densities (2.5 × 103, 5.0 × 103, and 1.0 × 104 cells/cm2). A difference was observed in the spread of N. bombycis Y9101 infection between low‐density and higher‐density cultures of host cells. The host cell density did not affect the productivity of secondary infective forms of the parasite. The principal factor determining the rate of microsporidian infection in vitro was the number of host cells existing within the reach of extruded short‐coiled polar tubes from spores germinated intracellularly.",
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T1 - Effects of Host Cell Density on Cell Infection Level in Antheraea eucalypti (Lepidoptera

T2 - Saturniidae) Cell Cultures Persistently Infected with Nosema bombycis (Microsporida: Nosematidae)

AU - Yasunaga-Aoki, Chisa

AU - FUNAKOSHI, MASAKO

AU - KAWARABATA, TAKESHI

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N2 - ABSTRACT. Spores of Nosema bombycis Y9101, isolated from the beet armyworm, Spodoptera exigua, were primed with an alkaline solution and inoculated into Antheraea eucalypti cell cultures. Infected cells were subcultured every five days at three cell densities (2.5 × 103, 5.0 × 103, and 1.0 × 104 cells/cm2). A difference was observed in the spread of N. bombycis Y9101 infection between low‐density and higher‐density cultures of host cells. The host cell density did not affect the productivity of secondary infective forms of the parasite. The principal factor determining the rate of microsporidian infection in vitro was the number of host cells existing within the reach of extruded short‐coiled polar tubes from spores germinated intracellularly.

AB - ABSTRACT. Spores of Nosema bombycis Y9101, isolated from the beet armyworm, Spodoptera exigua, were primed with an alkaline solution and inoculated into Antheraea eucalypti cell cultures. Infected cells were subcultured every five days at three cell densities (2.5 × 103, 5.0 × 103, and 1.0 × 104 cells/cm2). A difference was observed in the spread of N. bombycis Y9101 infection between low‐density and higher‐density cultures of host cells. The host cell density did not affect the productivity of secondary infective forms of the parasite. The principal factor determining the rate of microsporidian infection in vitro was the number of host cells existing within the reach of extruded short‐coiled polar tubes from spores germinated intracellularly.

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