Effects of intracellular pH on high pressure-induced hemolysis of anion transport inhibitor-treated erythrocytes

Masaki Matsumoto, Takeo Yamaguchi, Shigeyuki Terada, Eiji Kimoto

研究成果: ジャーナルへの寄稿記事

5 引用 (Scopus)

抄録

Effects of anion transport inhibitors such as 4,4'-diisothiocyanostilbene-2,2'-disulfonate (DIDS) and 4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonate on hemolysis of human erythrocytes at 200 MPa were examined by changing intracellular pH (7.2-7.9). These inhibitors suppressed the hemolysis at neutral pH but enhanced it at alkaline pH. However, such an enhancement was suppressed by cross-linking of membrane proteins using diamide. From the near-UV CD spectra of band 3 and the relation between hemolysis and anion transport in intact or trypsin-treated erythrocytes, it was found that such hemolytic properties were characterized by the binding of inhibitors to band 3. In addition, spectrin detachment from the erythrocyte membrane by high pressure was considerably suppressed by DIDS treatment at neutral pH, but not by DIDS labeling at alkaline pH. These results suggest that the interaction of the cytoplasmic domain of band 3 with the cytoskeleton, which is induced by the binding of ligands to the exofacial domain of band 3, is dependent on the intracellular pH, i.e., the linking is tightened at neutral pH but relaxed at alkaline pH.

元の言語英語
ページ(範囲)243-250
ページ数8
ジャーナルBiochimica et Biophysica Acta - Biomembranes
1280
発行部数2
DOI
出版物ステータス出版済み - 4 26 1996

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Hemolysis
Anions
Erythrocytes
Diamide
Pressure
Spectrin
Trypsin
Labeling
Membrane Proteins
Ligands
Membranes
Erythrocyte Membrane
Cytoskeleton

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Cell Biology

これを引用

Effects of intracellular pH on high pressure-induced hemolysis of anion transport inhibitor-treated erythrocytes. / Matsumoto, Masaki; Yamaguchi, Takeo; Terada, Shigeyuki; Kimoto, Eiji.

:: Biochimica et Biophysica Acta - Biomembranes, 巻 1280, 番号 2, 26.04.1996, p. 243-250.

研究成果: ジャーナルへの寄稿記事

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