Effects of KCN, SHAM and oxygen concentrations on respiratory properties of purified mitochondria isolated from Ananas comosus (pineapple) and Kalanchoë daigremontiana

Hoang Thi Kim Hong, Akihiro Nose, Sakae Agarie

研究成果: ジャーナルへの寄稿学術誌査読

9 被引用数 (Scopus)

抄録

Effects of potassium cyanide (KCN), salicylhydroxamic acid (SHAM), and oxygen concentrations on mitochondrial respiration were investigated in purified mitochondria of a typical phosphoeno/pyruvate carboxykinase (PCK) crassulacean acid metabolism (CAM) plant Ananas comosus (pineapple) and a typical malic enzyme (ME)-CAM plant Kalanchoë daigremontiana. Mitochondria of A. comosus oxidized succinate and NADH faster than that of K. daigremontiana. Succinate and NADH oxidations in mitochondria of both species were partially inhibited by KCN and SHAM, indicating that these oxidations were connected to cytochrome and alternative pathways in their electron transport chain (ETC). NADH oxidation was more sensitive to KCN than succinate oxidation, suggesting that the ETC from NADH oxidation was less connected to the alternative pathway than that from succinate in mitochondria of both species. Concurrent oxidation of succinate and NADH resulted in much higher rates of cytochrome and alternative respirations than each individual oxidation alone in both species. NADH oxidation in A. comosus mitochondria was more connected to the cytochrome pathway, so A. comosus could produce much more ATP than K. daigremontiana. This capacity might be one of the fitting mechanisms of A. comosus to produce a sufficient amount of ATP for cytosolic PCK in the daytime. In addition, the reduction of oxygen concentrations decreased not only the cytochrome respiration, but also the alternative respiration on succinate oxidation in mitochondria of both species, and the decrease was greater in K. daigremontiana than in A. comosus.

本文言語英語
ページ(範囲)383-392
ページ数10
ジャーナルPlant Production Science
8
4
DOI
出版ステータス出版済み - 2005
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 農業および作物学

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