Effects of mechanical strain on differentiation of osteoblastic cells

Aritsune Matsui, Taisu L. Masuda, Takahisa Anada, Ichiro Takahashi, Fumihito Arai, Masamichi Takami, Ryutaro Kamijo, Toshio Fukuda, Seishi Echigo, Osamu Suzuki

研究成果: Chapter in Book/Report/Conference proceedingConference contribution

抄録

The present study was designed to investigate whether compressive mechanical strain affects osteoblastic cell differentiation. We developed a culture system with the multi channel that induces the mechanical stimuli with quantitative gradient strain on seeded cells during the culture. This culture system was a computer-driven device that the various mode of mechanical strains can be applied, using the pressurization or depressurization, to deform cells cultured on PDMS membrane. The mRNA expression levels of osteoblast-related genes, such as osterix, collagen type I, and osteopontin, determined by real- time PCR, decreased by the compressive strain. On the contrary, the expression of RANKL, a factor related to the induction of osteoclasts, increased in the compressive strain, while the expression of osteoprotegrin, a molecule that antagonizes the function of the RANKL, decreased in the same strain. It was suggested that the function of osteoblastic cells may be influenced by the compressive strain.

本文言語英語
ホスト出版物のタイトル2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics"
ページ475-480
ページ数6
DOI
出版ステータス出版済み - 12 1 2008
外部発表はい
イベント2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "System Cell Engineering by Multi-scale Manipulation" - Nagoya, 日本
継続期間: 11 6 200811 9 2008

出版物シリーズ

名前2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008

その他

その他2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "System Cell Engineering by Multi-scale Manipulation"
国/地域日本
CityNagoya
Period11/6/0811/9/08

All Science Journal Classification (ASJC) codes

  • コンピュータ ビジョンおよびパターン認識
  • 人間とコンピュータの相互作用
  • 電子工学および電気工学

フィンガープリント

「Effects of mechanical strain on differentiation of osteoblastic cells」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル