Effects of monocrotaline pyrrole on morphology, number and production of nitric oxide of cultured artery endothelial cells

W. Cheng, Z. Li, T. Koyama, M. Oike, Y. Ito

研究成果: ジャーナルへの寄稿記事

抄録

Experimental model of pulmonary hypertension has been created by exposing animals to monocrotaline pyrrole (MCTP), but the cause of pulmonary hypertension induced by MCTP is still controversial. In this study, cell culture and DAF-2 fluorescence technique were used to investigate the effects of MCTP on Cell surface area, cell number and production of nitric oxide (NO) in cultured calf pulmonary artery endothelial cells (CPAE) and bovine aorta . endothelial cells (BAEC). MCTP induced the cell enlargement from (46 ± 4) to (223 ± 27) μm2 and decreased the number of cells from (175 ± 9) × 106 L-1 to (49 ± 6) × 106 L-1 in CPAE. Acetylcholine induced production of NO, measured by DAF-2, was decreased significantly from (16.1 ± 1.3) % to (1.5 ± 1.1)% and from (14.2 ± 1.6)% to (1.4 ± 1.6) % in CPAE and BAEC, respectively. The results indicate that MCTP may damage endothelial cells and inhibit NO production, which maybe another pathogenesis of MCTP induced pulmonary hypertension.

元の言語英語
ページ(範囲)169-171
ページ数3
ジャーナルChinese Journal of Pharmacology and Toxicology
15
発行部数3
出版物ステータス出版済み - 1 1 2001

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Endothelial cells
Nitric Oxide
Endothelial Cells
Arteries
Pulmonary Hypertension
Pulmonary Artery
Cell Count
Cell Enlargement
Cell culture
Acetylcholine
Aorta
monocrotaline pyrrole
Animals
Theoretical Models
Cell Culture Techniques
Fluorescence

All Science Journal Classification (ASJC) codes

  • Toxicology
  • Pharmacology

これを引用

Effects of monocrotaline pyrrole on morphology, number and production of nitric oxide of cultured artery endothelial cells. / Cheng, W.; Li, Z.; Koyama, T.; Oike, M.; Ito, Y.

:: Chinese Journal of Pharmacology and Toxicology, 巻 15, 番号 3, 01.01.2001, p. 169-171.

研究成果: ジャーナルへの寄稿記事

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abstract = "Experimental model of pulmonary hypertension has been created by exposing animals to monocrotaline pyrrole (MCTP), but the cause of pulmonary hypertension induced by MCTP is still controversial. In this study, cell culture and DAF-2 fluorescence technique were used to investigate the effects of MCTP on Cell surface area, cell number and production of nitric oxide (NO) in cultured calf pulmonary artery endothelial cells (CPAE) and bovine aorta . endothelial cells (BAEC). MCTP induced the cell enlargement from (46 ± 4) to (223 ± 27) μm2 and decreased the number of cells from (175 ± 9) × 106 L-1 to (49 ± 6) × 106 L-1 in CPAE. Acetylcholine induced production of NO, measured by DAF-2, was decreased significantly from (16.1 ± 1.3) {\%} to (1.5 ± 1.1){\%} and from (14.2 ± 1.6){\%} to (1.4 ± 1.6) {\%} in CPAE and BAEC, respectively. The results indicate that MCTP may damage endothelial cells and inhibit NO production, which maybe another pathogenesis of MCTP induced pulmonary hypertension.",
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AU - Oike, M.

AU - Ito, Y.

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