TY - JOUR
T1 - Effects of nitric oxide on ammonia decomposition by hepatocytes under shear stress
AU - Sumii, Tateki
AU - Nakano, Yohei
AU - Abe, Takuma
AU - Nakashima, Kazuhiro
AU - Sera, Toshihiro
AU - Kudo, Susumu
N1 - Funding Information:
This study was supported in part by a Grant-in-Aid for JSPS Fellows (255172). We would like to thank Hiroshi Sakamoto and Shinichiro Doi for their technical assistance.
Publisher Copyright:
© Japanese Society of Biorheology 2016.
PY - 2016
Y1 - 2016
N2 - Nitric oxide (NO) and shear stress modulates hepatocyte functions, including ammonia metabolism. This study investigated the simultaneous effects of NO and shear stress on hepatocyte functions. We developed a cell culture device to simultaneously apply NO and shear stress to hepatocytes, and measured changes in ammonia decomposition by hepatocytes in response to changes in NO concentration and shear stress. NO was supplied directly to cells at a constant rate at 0, 0.5, 5, and 25 ppm, and shear stress was either applied at 0.6 Pa or not (static culture). Ammonia decomposition in static culture was higher under all NO loads compared with 0 ppm NO, and was highest under 0.5 ppm NO and decreased under higher NO loads. In the absence of NO load, ammonia decomposition under shear stress was approximately double that in static culture. Under the simultaneous application of NO and shear stress load, ammonia decomposition under 0.5 ppm NO was approximately twice as high as under 0 ppm NO, but was almost the same under 25 ppm NO as under 0 ppm NO. These results indicate that both NO and shear stress enhance ammonia decomposition although the enhancement depends on the NO concentration in their immediate surroundings.
AB - Nitric oxide (NO) and shear stress modulates hepatocyte functions, including ammonia metabolism. This study investigated the simultaneous effects of NO and shear stress on hepatocyte functions. We developed a cell culture device to simultaneously apply NO and shear stress to hepatocytes, and measured changes in ammonia decomposition by hepatocytes in response to changes in NO concentration and shear stress. NO was supplied directly to cells at a constant rate at 0, 0.5, 5, and 25 ppm, and shear stress was either applied at 0.6 Pa or not (static culture). Ammonia decomposition in static culture was higher under all NO loads compared with 0 ppm NO, and was highest under 0.5 ppm NO and decreased under higher NO loads. In the absence of NO load, ammonia decomposition under shear stress was approximately double that in static culture. Under the simultaneous application of NO and shear stress load, ammonia decomposition under 0.5 ppm NO was approximately twice as high as under 0 ppm NO, but was almost the same under 25 ppm NO as under 0 ppm NO. These results indicate that both NO and shear stress enhance ammonia decomposition although the enhancement depends on the NO concentration in their immediate surroundings.
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U2 - 10.17106/jbr.30.19
DO - 10.17106/jbr.30.19
M3 - Article
AN - SCOPUS:84975673391
VL - 30
SP - 19
EP - 26
JO - Journal of Biorheology
JF - Journal of Biorheology
SN - 1867-0466
IS - 1
ER -