Effects of oil drops containing Lactobacillus salivarius WB21 on periodontal health and oral microbiota producing volatile sulfur compounds

Nao Suzuki, Kazunari Tanabe, Toru Takeshita, Masahiro Yoneda, Tomoyuki Iwamoto, Sueko Oshiro, Yoshihisa Yamashita, Takao Hirofuji

研究成果: ジャーナルへの寄稿記事

14 引用 (Scopus)

抄録

The objective of this paper is to evaluate the effects of oil drops containing Lactobacillus salivarius WB21 on periodontal health and oral microbiota producing volatile sulfur compounds (VSCs). For this study, 42 subjects were randomly assigned to receive oil samples containing L. salivarius WB21 or a placebo for two weeks. Oral assessment and saliva collection were performed on days 1 and 15. Bacterial analysis was performed using the real-time polymerase chain reaction and terminal restriction fragment length polymorphism (T-RFLP). In both the experimental and placebo groups, the average probing depth, number of periodontal pockets, and the percentage of bleeding on probing (BOP) decreased while stimulated salivary flow increased on day 15. BOP was reduced in the experimental group compared with the placebo group (P = 0.010). In the experimental group, total bacterial numbers decreased, and the number of L. salivarius increased. The number of Prevotella intermedia, which is correlated with hydrogen sulfide concentration in mouth air, increased in the placebo group and did not change in the experimental group. T-RFLP analysis found that the peak area proportions representing Porphyromonas gingivalis, P. intermedia, Tannerella forsythensis, and Fusobacterium nucleatum decreased in the experimental group, although there was no significant change in the bacterial composition. Thus we observed oil drops containing L. salivarius WB21 improved BOP and inhibited the reproduction of total and VSC-producing periodontopathic bacteria compared with the placebo group, but also showed the limit of its efficacy in controlling VSCs producing and periodontal pathogens.

元の言語英語
記事番号017106
ジャーナルJournal of Breath Research
6
発行部数1
DOI
出版物ステータス出版済み - 3 1 2012

Fingerprint

Sulfur Compounds
Microbiota
Oral Health
Oils
Placebos
Prevotella intermedia
Hemorrhage
Restriction Fragment Length Polymorphisms
Fusobacterium nucleatum
Periodontal Pocket
Porphyromonas gingivalis
Hydrogen Sulfide
Saliva
Reproduction
Mouth
Real-Time Polymerase Chain Reaction
Air
Lactobacillus salivarius
Bacteria

All Science Journal Classification (ASJC) codes

  • Pulmonary and Respiratory Medicine

これを引用

Effects of oil drops containing Lactobacillus salivarius WB21 on periodontal health and oral microbiota producing volatile sulfur compounds. / Suzuki, Nao; Tanabe, Kazunari; Takeshita, Toru; Yoneda, Masahiro; Iwamoto, Tomoyuki; Oshiro, Sueko; Yamashita, Yoshihisa; Hirofuji, Takao.

:: Journal of Breath Research, 巻 6, 番号 1, 017106, 01.03.2012.

研究成果: ジャーナルへの寄稿記事

Suzuki, Nao ; Tanabe, Kazunari ; Takeshita, Toru ; Yoneda, Masahiro ; Iwamoto, Tomoyuki ; Oshiro, Sueko ; Yamashita, Yoshihisa ; Hirofuji, Takao. / Effects of oil drops containing Lactobacillus salivarius WB21 on periodontal health and oral microbiota producing volatile sulfur compounds. :: Journal of Breath Research. 2012 ; 巻 6, 番号 1.
@article{767bfbf71a2c4e7e9c7ca6d28701bc33,
title = "Effects of oil drops containing Lactobacillus salivarius WB21 on periodontal health and oral microbiota producing volatile sulfur compounds",
abstract = "The objective of this paper is to evaluate the effects of oil drops containing Lactobacillus salivarius WB21 on periodontal health and oral microbiota producing volatile sulfur compounds (VSCs). For this study, 42 subjects were randomly assigned to receive oil samples containing L. salivarius WB21 or a placebo for two weeks. Oral assessment and saliva collection were performed on days 1 and 15. Bacterial analysis was performed using the real-time polymerase chain reaction and terminal restriction fragment length polymorphism (T-RFLP). In both the experimental and placebo groups, the average probing depth, number of periodontal pockets, and the percentage of bleeding on probing (BOP) decreased while stimulated salivary flow increased on day 15. BOP was reduced in the experimental group compared with the placebo group (P = 0.010). In the experimental group, total bacterial numbers decreased, and the number of L. salivarius increased. The number of Prevotella intermedia, which is correlated with hydrogen sulfide concentration in mouth air, increased in the placebo group and did not change in the experimental group. T-RFLP analysis found that the peak area proportions representing Porphyromonas gingivalis, P. intermedia, Tannerella forsythensis, and Fusobacterium nucleatum decreased in the experimental group, although there was no significant change in the bacterial composition. Thus we observed oil drops containing L. salivarius WB21 improved BOP and inhibited the reproduction of total and VSC-producing periodontopathic bacteria compared with the placebo group, but also showed the limit of its efficacy in controlling VSCs producing and periodontal pathogens.",
author = "Nao Suzuki and Kazunari Tanabe and Toru Takeshita and Masahiro Yoneda and Tomoyuki Iwamoto and Sueko Oshiro and Yoshihisa Yamashita and Takao Hirofuji",
year = "2012",
month = "3",
day = "1",
doi = "10.1088/1752-7155/6/1/017106",
language = "English",
volume = "6",
journal = "Journal of Breath Research",
issn = "1752-7155",
publisher = "IOP Publishing Ltd.",
number = "1",

}

TY - JOUR

T1 - Effects of oil drops containing Lactobacillus salivarius WB21 on periodontal health and oral microbiota producing volatile sulfur compounds

AU - Suzuki, Nao

AU - Tanabe, Kazunari

AU - Takeshita, Toru

AU - Yoneda, Masahiro

AU - Iwamoto, Tomoyuki

AU - Oshiro, Sueko

AU - Yamashita, Yoshihisa

AU - Hirofuji, Takao

PY - 2012/3/1

Y1 - 2012/3/1

N2 - The objective of this paper is to evaluate the effects of oil drops containing Lactobacillus salivarius WB21 on periodontal health and oral microbiota producing volatile sulfur compounds (VSCs). For this study, 42 subjects were randomly assigned to receive oil samples containing L. salivarius WB21 or a placebo for two weeks. Oral assessment and saliva collection were performed on days 1 and 15. Bacterial analysis was performed using the real-time polymerase chain reaction and terminal restriction fragment length polymorphism (T-RFLP). In both the experimental and placebo groups, the average probing depth, number of periodontal pockets, and the percentage of bleeding on probing (BOP) decreased while stimulated salivary flow increased on day 15. BOP was reduced in the experimental group compared with the placebo group (P = 0.010). In the experimental group, total bacterial numbers decreased, and the number of L. salivarius increased. The number of Prevotella intermedia, which is correlated with hydrogen sulfide concentration in mouth air, increased in the placebo group and did not change in the experimental group. T-RFLP analysis found that the peak area proportions representing Porphyromonas gingivalis, P. intermedia, Tannerella forsythensis, and Fusobacterium nucleatum decreased in the experimental group, although there was no significant change in the bacterial composition. Thus we observed oil drops containing L. salivarius WB21 improved BOP and inhibited the reproduction of total and VSC-producing periodontopathic bacteria compared with the placebo group, but also showed the limit of its efficacy in controlling VSCs producing and periodontal pathogens.

AB - The objective of this paper is to evaluate the effects of oil drops containing Lactobacillus salivarius WB21 on periodontal health and oral microbiota producing volatile sulfur compounds (VSCs). For this study, 42 subjects were randomly assigned to receive oil samples containing L. salivarius WB21 or a placebo for two weeks. Oral assessment and saliva collection were performed on days 1 and 15. Bacterial analysis was performed using the real-time polymerase chain reaction and terminal restriction fragment length polymorphism (T-RFLP). In both the experimental and placebo groups, the average probing depth, number of periodontal pockets, and the percentage of bleeding on probing (BOP) decreased while stimulated salivary flow increased on day 15. BOP was reduced in the experimental group compared with the placebo group (P = 0.010). In the experimental group, total bacterial numbers decreased, and the number of L. salivarius increased. The number of Prevotella intermedia, which is correlated with hydrogen sulfide concentration in mouth air, increased in the placebo group and did not change in the experimental group. T-RFLP analysis found that the peak area proportions representing Porphyromonas gingivalis, P. intermedia, Tannerella forsythensis, and Fusobacterium nucleatum decreased in the experimental group, although there was no significant change in the bacterial composition. Thus we observed oil drops containing L. salivarius WB21 improved BOP and inhibited the reproduction of total and VSC-producing periodontopathic bacteria compared with the placebo group, but also showed the limit of its efficacy in controlling VSCs producing and periodontal pathogens.

UR - http://www.scopus.com/inward/record.url?scp=84857593500&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84857593500&partnerID=8YFLogxK

U2 - 10.1088/1752-7155/6/1/017106

DO - 10.1088/1752-7155/6/1/017106

M3 - Article

C2 - 22368259

AN - SCOPUS:84857593500

VL - 6

JO - Journal of Breath Research

JF - Journal of Breath Research

SN - 1752-7155

IS - 1

M1 - 017106

ER -