TY - JOUR
T1 - Effects of plasma-activated Ringer’s lactate solution on cancer cells
T2 - evaluation of genotoxicity
AU - Liu, Yang
AU - Nakatsu, Yoshimichi
AU - Tanaka, Hiromasa
AU - Koga, Kazunori
AU - Ishikawa, Kenji
AU - Shiratani, Masaharu
AU - Hori, Masaru
N1 - Funding Information:
The authors thank the members of Medical Biophysics and Radiation Biology, Department of Basic Medicine, Faculty of Medical Sciences, Kyushu University, for their support. This work was partly supported by the DII Collaborative Graduate Program for Accelerating Innovation in Future Electronics, Nagoya University.
Funding Information:
This study was supported in part by a Grant-in-Aid for Specially Promoted Research: Development of “super-bio-functions” by plasma-activated biological substances. (KAKENHI 19H05462).
Publisher Copyright:
© 2023, The Author(s).
PY - 2023/12
Y1 - 2023/12
N2 - Background: Non-thermal atmospheric pressure plasma technologies form the core of many scientific advances, including in the electronic, industrial, and biotechnological fields. The use of plasma as a cancer therapy has recently attracted significant attention due to its cancer cell killing activity. Plasma-activated Ringer’s lactate solution (PAL) exhibits such activity. In addition to ROS, PAL contains active compounds or species that cause cancer cell death, but the potential mutagenic risks of PAL have not been studied. Results: PAL has a low pH value and a high concentration of H2O2. H2O2 was removed from PAL using catalase and catalase-treated PAL with a pH of 5.9 retained a killing effect on HeLa cells whereas this effect was not observed if the PAL was adjusted to pH 7.2. Catalase-treated PAL at pH 5.9 had no significant effect on mutation frequency, the expression of γH2AX, or G2 arrest in HeLa cells. Conclusion: PAL contains one or more active compounds or species in addition to H2O2 that have a killing effect on HeLa cells. The compound(s) is active at lower pH conditions and apparently exhibits no genotoxicity. This study suggested that identification of the active compound(s) in PAL could lead to the development of novel anticancer drugs for future cancer therapy.
AB - Background: Non-thermal atmospheric pressure plasma technologies form the core of many scientific advances, including in the electronic, industrial, and biotechnological fields. The use of plasma as a cancer therapy has recently attracted significant attention due to its cancer cell killing activity. Plasma-activated Ringer’s lactate solution (PAL) exhibits such activity. In addition to ROS, PAL contains active compounds or species that cause cancer cell death, but the potential mutagenic risks of PAL have not been studied. Results: PAL has a low pH value and a high concentration of H2O2. H2O2 was removed from PAL using catalase and catalase-treated PAL with a pH of 5.9 retained a killing effect on HeLa cells whereas this effect was not observed if the PAL was adjusted to pH 7.2. Catalase-treated PAL at pH 5.9 had no significant effect on mutation frequency, the expression of γH2AX, or G2 arrest in HeLa cells. Conclusion: PAL contains one or more active compounds or species in addition to H2O2 that have a killing effect on HeLa cells. The compound(s) is active at lower pH conditions and apparently exhibits no genotoxicity. This study suggested that identification of the active compound(s) in PAL could lead to the development of novel anticancer drugs for future cancer therapy.
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U2 - 10.1186/s41021-023-00260-x
DO - 10.1186/s41021-023-00260-x
M3 - Article
AN - SCOPUS:85146275523
SN - 1880-7046
VL - 45
JO - Genes and Environment
JF - Genes and Environment
IS - 1
M1 - 3
ER -
