Effects of positive pressure in odontogenic keratocysts

S. Oka, Y. Kubota, T. Yamashiro, S. Ogata, T. Ninomiya, S. Ito, K. Shirasuna

研究成果: Contribution to journalArticle査読

30 被引用数 (Scopus)

抄録

Intracystic fluid pressure is thought to be involved in odontogenic cyst growth. In this study, we investigated the effects of positive pressure on the expression of interleukin-1α (IL-1α), matrix metalloproteinases (MMPs), and prostaglandin E2 (PGE2) in odontogenic keratocysts to determine whether this pressure stimulates inflammatory cytokine production and signaling of osteoclastogenic events. Positive pressure enhanced the expression of IL-1α mRNA and protein in odontogenic keratocyst epithelial cells, and increased the secretion of MMP-1, MMP-2, MMP-3, and PGE2 in a co-culture of odontogenic keratocyst fibroblasts and the epithelial cells. The pressure-induced secretions were inhibited by an interleukin-1 receptor antagonist. Recombinant human interleukin-1α (rhIL-1α) increased the secretion of MMP-1, MMP-2, MMP-3, and PGE 2 in the fibroblasts. Furthermore, in the fibroblasts, rhIL-1α enhanced the expression of macrophage colony-stimulating factor (M-CSF) mRNA, and rhIL-1α-induced PGE2 increased the expression of nuclear factor κB ligand (RANKL) mRNA. Thus, positive pressure may play a crucial role in odontogenic keratocyst growth via stimulating the expression of IL-1α in epithelial cells.

本文言語英語
ページ(範囲)913-918
ページ数6
ジャーナルJournal of Dental Research
84
10
DOI
出版ステータス出版済み - 10 2005

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

フィンガープリント 「Effects of positive pressure in odontogenic keratocysts」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル